School of Pharmacy and Pharmaceutical Sciences, Department of NanoEngineering, University of California at San Diego, La Jolla, California 92093, USA.
Anal Chem. 2011 May 15;83(10):3943-9. doi: 10.1021/ac200591a. Epub 2011 Apr 20.
A fiber-optic coupled attenuated total reflection (ATR)-FT-IR spectroscopy technique was applied to the study of two different therapeutic delivery systems, acid degradable hydrogels and nanoparticles. Real time exponential release of a model protein, human serum albumin (HSA), was observed from two different polymeric hydrogels formulated with a pH sensitive cross-linker. Spectroscopic examination of nanoparticles formulated with an acid degradable polymer shell and encapsulated HSA exhibited vibrational signatures characteristic of both particle and payload when exposed to lowered pH conditions, demonstrating the ability of this methodology to simultaneously measure phenomena arising from a system with a mixture of components. In addition, thorough characterization of these pH sensitive delivery vehicles without encapsulated protein was also accomplished in order to separate the effects of the payload during degradation. When in situ, real time detection in combination with the ability to specifically identify different components in a mixture without involved sample preparation and minimal sample disturbance is provided, the versatility and suitability of this type of experiment for research in the pharmaceutical field is demonstrated.
一种光纤耦合衰减全反射（ATR）-FT-IR 光谱技术被应用于两种不同的治疗药物输送系统的研究，即酸降解水凝胶和纳米粒子。通过实时监测，观察到两种不同的聚合物水凝胶中模型蛋白（人血清白蛋白，HSA）的指数释放，这两种水凝胶都使用了 pH 敏感的交联剂。对具有酸降解聚合物外壳和包封 HSA 的纳米粒子进行光谱检查时，发现当暴露于较低 pH 值条件下时，其具有特征性的振动信号，表明该方法能够同时测量具有混合成分的系统中出现的现象。此外，还对未包封蛋白的这些 pH 敏感输送载体进行了彻底的表征，以便在降解过程中分离出有效载荷的影响。当实时原位检测与无需涉及样品制备和最小样品干扰的混合物中特定识别不同成分的能力相结合时，这种类型的实验在药物研究领域的多功能性和适用性得到了证明。
