Grunelius-Moellgaard Laboratory, Department of Botany and Molecular Evolution, Senckenberg Research Institute, Senckenberganlage 25, D-60325 Frankfurt, Germany.
Mol Ecol Resour. 2011 May;11(3):450-60. doi: 10.1111/j.1755-0998.2011.02984.x. Epub 2011 Feb 6.
There has been considerable debate regarding locus choice for DNA barcoding land plants. This is partly attributable to a shortage of comparable data from proposed candidate loci on a common set of samples. In this study, we evaluated main candidate plastid regions (rpoC1, rpoB, accD) and additional plastid markers (psbB, psbN, psbT exons and the trnS-trnG spacer) as well as the nuclear ribosomal spacer region (ITS1-5.8S-ITS2) in a group of land plants belonging to the mahogany family, Meliaceae. Across these samples, only ITS showed high levels of resolvability. Interspecific sharing of sequences from individual plastid loci was common. The combination of multiple loci did not improve performance. DNA barcoding with ITS alone revealed cryptic species and proved useful in identifying species listed in Convention on International Trade of Endangered Species appendixes.
对于陆地植物 DNA 条形码的基因座选择，存在大量争议。这部分归因于在共同的样本集中，来自拟议候选基因座的可比数据不足。在这项研究中，我们评估了主要候选质体区域（rpoC1、rpoB、accD）和其他质体标记（psbB、psbN、psbT 外显子和 trnS-trnG 间隔区）以及核核糖体间隔区（ITS1-5.8S-ITS2）在属于桃花心木科的一组陆地植物中。在这些样本中，只有 ITS 显示出高分辨率。来自单个质体基因座的序列在种间共享很常见。多个基因座的组合并没有提高性能。仅使用 ITS 进行 DNA 条形码显示出隐种，并有助于识别列入《濒危物种国际贸易公约》附录的物种。
