Key Laboratory of Macrocyclic and Supramolecular Chemistry of Guizhou Province, Guizhou University, Guiyang 550025, China.
Talanta. 2011 May 15;84(3):684-9. doi: 10.1016/j.talanta.2011.01.071. Epub 2011 Feb 4.
The supramolecular interaction of cucurbit(n=7)uril (Q[7]) with berberine chloride (BER) has been studied in aqueous solution at pH 2.0 and room temperature by spectro-fluorimetry. The association constant of the complex was 2.07 × 10(6) L mol(-1) calculated by using a nonlinear least squares method. (1)H NMR spectra confirmed that a 1:1 stable complex is formed between Q[7] and BER. This work proposes a possible interaction mode, in which the guest BER is incorporated inside the hydrophobic cavity of the host Q[7] via the isoquinoline ring part of the guest molecule. Based on a significant enhancement of the fluorescence intensity of this supramolecular complex, a spectrofluorimetric method with high sensitivity and selectivity has been developed for the determination of BER in aqueous solution in the presence of Q[7]. The linear range of the method was from 7.43 to 11.2 × 10(3) ng mL(-1)with the detection limit 4.2 ng mL(-1). There was no interference from the compounds normally used in tablets, serum or urine constituents. The proposed method was applied to the determination of BER in tablets, serum and urine samples with satisfactory results and good consistency with those obtained by the pharmacopoeia method. This shows that it has promising potential for therapeutic drug monitoring and pharmacokinetics and for clinical application.
在 pH 2.0 和室温下，通过光谱荧光法研究了葫芦（n=7）脲（Q[7]）与盐酸小檗碱（BER）在水溶液中的超分子相互作用。通过非线性最小二乘法计算，该配合物的结合常数为 2.07×10(6)L mol(-1)。（1）H NMR 光谱证实了 Q[7]和 BER 之间形成了 1:1 的稳定配合物。这项工作提出了一种可能的相互作用模式，其中客体 BER 通过客体分子的异喹啉环部分被包含在主体 Q[7]的疏水性腔中。基于这种超分子配合物荧光强度的显著增强，建立了一种在 Q[7]存在下测定水溶液中 BER 的高灵敏度和选择性荧光分光光度法。该方法的线性范围为 7.43 至 11.2×10(3)ng mL(-1)，检测限为 4.2 ng mL(-1)。通常在片剂、血清或尿液成分中使用的化合物没有干扰。该方法已应用于片剂、血清和尿液样品中 BER 的测定，结果令人满意，与药典方法所得结果具有良好的一致性。这表明它在治疗药物监测和药代动力学以及临床应用方面具有广阔的应用前景。
