Department of Neuroscience, Dr Molewaterplein 50, Erasmus MC, 3015 GE Rotterdam, The Netherlands.
Neuron. 2011 Apr 14;70(1):43-50. doi: 10.1016/j.neuron.2011.02.044.
Long-term depression at parallel fiber-Purkinje cell synapses (PF-PC LTD) has been proposed to be required for cerebellar motor learning. To date, tests of this hypothesis have sought to interfere with receptors (mGluR1) and enzymes (PKC, PKG, or αCamKII) necessary for induction of PF-PC LTD and thereby determine if cerebellar motor learning is impaired. Here, we tested three mutant mice that target the expression of PF-PC LTD by blocking internalization of AMPA receptors. Using three different cerebellar coordination tasks (adaptation of the vestibulo-ocular reflex, eyeblink conditioning, and locomotion learning on the Erasmus Ladder), we show that there is no motor learning impairment in these mutant mice that lack PF-PC LTD. These findings demonstrate that PF-PC LTD is not essential for cerebellar motor learning.
长时程抑制在平行纤维-浦肯野细胞突触（PF-PC LTD）被提出是小脑运动学习所必需的。迄今为止，对这一假说的检验试图干扰诱导 PF-PC LTD 所必需的受体（mGluR1）和酶（PKC、PKG 或 αCamKII），从而确定小脑运动学习是否受损。在这里，我们测试了三种突变小鼠，通过阻断 AMPA 受体的内化来靶向 PF-PC LTD 的表达。使用三种不同的小脑协调任务（前庭眼反射的适应、眨眼条件反射和在 Erasmus 梯上的运动学习），我们表明这些缺乏 PF-PC LTD 的突变小鼠没有运动学习障碍。这些发现表明，PF-PC LTD 对于小脑运动学习不是必需的。
