Ministry of Education Key Laboratory for the Green Preparation and Application of Functional Materials, Hubei University, Wuhan 430062, China.
Biomaterials. 2011 Jul;32(21):4976-86. doi: 10.1016/j.biomaterials.2011.03.050. Epub 2011 Apr 12.
Promising drug nanocarriers consisting of mono-dispersed and pH sensitive chitosan-silica hollow nanospheres (CS-SiO(2) HNPs) suitable for breast cancer therapy are produced and investigated. The SiO(2) HNPs are fabricated using a one-step, one-medium process which obviates the need for post-treatment to remove the templates, additional dissolution, or calcination. Taking advantage of the cross-linking reaction with (3-Glycidyloxypropyl) trimethoxysilane (GTPMS), cationic polysaccharide-chitosan decorates the surface and produces pH sensitive CS-SiO(2) HNPs. The materials enable controlled release of loaded drugs in pericellular and interstitial environments. In particular, the antibody molecule (to ErbB 2) can be conjugated onto the surface of the CS-SiO(2) HNPs thereby allowing the hollow nanospheres to serve as a targeted delivery agent to breast cancer cells. TNF-α are delivered to MCF-7 breast cancer cells under both in vitro and in vivo conditions to suppress the growth of cancerous cells and even kill them with high therapeutic efficacy. Owing to their hollow inner cavity and porous structures, the CS-SiO(2) HNPs are excellent pH-responsive targeted nanocarriers.
制备并研究了由单分散且 pH 敏感的壳聚糖-二氧化硅中空纳米球（CS-SiO2 HNPs）组成的有前途的药物纳米载体，适用于乳腺癌治疗。SiO2 HNPs 采用一步法和单介质工艺制备，避免了后处理去除模板、额外溶解或煅烧的需要。利用（3-缩水甘油醚丙基）三甲氧基硅烷（GTPMS）的交联反应，阳离子多糖壳聚糖修饰表面并产生 pH 敏感的 CS-SiO2 HNPs。这些材料能够在细胞外和细胞间隙环境中控制载药的释放。特别是，抗体分子（针对 ErbB2）可以连接到 CS-SiO2 HNPs 的表面，从而使中空纳米球能够作为靶向递药剂递送到乳腺癌细胞。TNF-α 被递送到 MCF-7 乳腺癌细胞中，无论是在体外还是体内条件下，都能抑制癌细胞的生长，甚至以高治疗效果杀死它们。由于其空心内腔和多孔结构，CS-SiO2 HNPs 是出色的 pH 响应性靶向纳米载体。
