肌 2 变化和组织损伤后下坡跑在 mdx 小鼠。
Changes in muscle T2 and tissue damage after downhill running in mdx mice.
机构信息
Department of Physical Therapy, University of Florida, Gainesville, Florida, USA.
出版信息
Muscle Nerve. 2011 Jun;43(6):878-86. doi: 10.1002/mus.21986. Epub 2011 Apr 12.
Abstract
INTRODUCTION
In this study we compared the effects of downhill or horizontal treadmill running on the magnetic resonance imaging (MRI) transverse relaxation time constant (T(2)) in mdx mice.
METHODS
Mice underwent either downhill (n = 11 mdx, n = 6 controls) or horizontal running (n = 9, mdx only) on a treadmill. MRI was conducted prior to exercise, immediately afterward (∽20 minutes), and then 24 and 48 hours after exercise.
RESULTS
A higher percentage of pixels with elevated T(2) in the lower hindlimb muscles was observed in the mdx mice compared with controls both pre-exercise (P < 0.001) and at each time-point after downhill running (P < 0.05), but not with horizontal running. The medial compartment muscles appeared to be the most susceptible to increased T(2).
CONCLUSIONS
Downhill running provides a stimulus for inducing acute changes in muscle T(2) in mdx mice. MRI is a non-invasive approach for examining acute muscle damage and recovery in multiple muscle groups simultaneously.
摘要
简介
在这项研究中，我们比较了下坡或水平跑步机跑步对 mdx 小鼠磁共振成像（MRI）横向弛豫时间常数（T（2））的影响。
方法
小鼠在跑步机上进行下坡（mdx n = 11，对照组 n = 6）或水平跑步（仅 mdx n = 9）。在运动前、运动后即刻（约 20 分钟）以及运动后 24 小时和 48 小时进行 MRI。
结果
与对照组相比，mdx 小鼠在运动前（P < 0.001）和下坡运动后的每个时间点（P < 0.05），其较低肢肌肉中 T（2）升高的像素百分比更高，但在水平跑步时则没有。内侧间隔肌肉似乎最容易受到 T（2）增加的影响。
结论
下坡跑步为诱导 mdx 小鼠肌肉 T（2）急性变化提供了刺激。MRI 是一种非侵入性方法，可同时检查多个肌肉群的急性肌肉损伤和恢复情况。
相似文献
1
Changes in muscle T2 and tissue damage after downhill running in mdx mice.肌 2 变化和组织损伤后下坡跑在 mdx 小鼠。
Muscle Nerve. 2011 Jun;43(6):878-86. doi: 10.1002/mus.21986. Epub 2011 Apr 12.
2
Effects of PDE5 inhibition on dystrophic muscle following an acute bout of downhill running and endurance training.磷酸二酯酶5抑制对下坡跑和耐力训练急性发作后营养不良肌肉的影响。
J Appl Physiol (1985). 2019 Jun 1;126(6):1737-1745. doi: 10.1152/japplphysiol.00664.2018. Epub 2019 Apr 4.
3
Effects of muscle damage on phosphorus magnetic resonance spectroscopy indices of energetic status and sarcolemma integrity in young mdx mice.肌肉损伤对年轻 mdx 小鼠能量状态和肌细胞膜完整性的磷磁共振波谱指标的影响。
NMR Biomed. 2022 Mar;35(3):e4659. doi: 10.1002/nbm.4659. Epub 2021 Nov 28.
4
Muscle fibers of mdx mice are more vulnerable to exercise than those of normal mice.与正常小鼠相比，mdx小鼠的肌纤维对运动更敏感。
Neuromuscul Disord. 1997 Dec;7(8):487-92. doi: 10.1016/s0960-8966(97)00115-6.
5
Chronic exercise accelerates the degeneration-regeneration cycle and downregulates insulin-like growth factor-1 in muscle of mdx mice.长期运动加速mdx小鼠肌肉中的退化-再生循环并下调胰岛素样生长因子-1。
Muscle Nerve. 2005 Aug;32(2):191-9. doi: 10.1002/mus.20351.
6
Recovery of damaged skeletal muscle in mdx mice through low-intensity endurance exercise.通过低强度耐力运动恢复 mdx 小鼠受损的骨骼肌。
Int J Sports Med. 2014 Jan;35(1):19-27. doi: 10.1055/s-0033-1343405. Epub 2013 Jul 18.
7
Duchenne muscular dystrophy progression induced by downhill running is accompanied by increased endomysial fibrosis and oxidative damage DNA in muscle of mdx mice.Duchenne 型肌营养不良症进展诱导的下坡跑伴随着 mdx 小鼠肌肉内的肌内膜纤维化和氧化损伤 DNA 的增加。
J Mol Histol. 2023 Feb;54(1):41-54. doi: 10.1007/s10735-022-10109-2. Epub 2022 Nov 8.
8
Effects of aging and voluntary exercise on the function of dystrophic muscle from mdx mice.衰老和自愿运动对mdx小鼠营养不良性肌肉功能的影响。
Am J Phys Med Rehabil. 1998 Jan-Feb;77(1):20-7. doi: 10.1097/00002060-199801000-00004.
9
Adaptive and nonadaptive responses to voluntary wheel running by mdx mice.mdx小鼠对自愿性转轮运动的适应性和非适应性反应。
Muscle Nerve. 2008 Oct;38(4):1290-303. doi: 10.1002/mus.21141.
10
Normal myoblast implantation in MDX mice prevents muscle damage by exercise.正常成肌细胞植入MDX小鼠可预防运动引起的肌肉损伤。
Biochem Biophys Res Commun. 1998 Sep 18;250(2):321-7. doi: 10.1006/bbrc.1998.9276.
引用本文的文献
1
A novel ex vivo protocol that mimics length and excitation changes of human muscles during walking induces force losses in EDL but not in soleus of mdx mice.一种模拟人类行走过程中肌肉长度和兴奋变化的新型离体实验方案，可导致mdx小鼠的趾长伸肌（EDL）出现力量损失，但比目鱼肌未出现力量损失。
PLoS One. 2025 Apr 7;20(4):e0320901. doi: 10.1371/journal.pone.0320901. eCollection 2025.
2
Advanced quantitative magnetic resonance imaging of lower extremity muscle microtrauma after marathon: a mini review.马拉松赛后下肢肌肉微创伤的高级定量磁共振成像：一篇综述
Front Sports Act Living. 2024 Oct 29;6:1481731. doi: 10.3389/fspor.2024.1481731. eCollection 2024.
3
Distribution of MRI-derived T2 values as a biomarker for in vivo rapid screening of phenotype severity in mdx mice.作为一种生物标志物，基于 MRI 的 T2 值分布可用于体内快速筛选 mdx 小鼠的表型严重程度。
PLoS One. 2024 Sep 19;19(9):e0310551. doi: 10.1371/journal.pone.0310551. eCollection 2024.
4
Duchenne muscular dystrophy disease severity impacts skeletal muscle progenitor cells systemic delivery.杜氏肌营养不良症的疾病严重程度会影响骨骼肌祖细胞的全身递送。
Front Physiol. 2023 May 9;14:1190524. doi: 10.3389/fphys.2023.1190524. eCollection 2023.
5
Magnetic resonance quantification of skeletal muscle lipid infiltration in a humanized mouse model of Duchenne muscular dystrophy.磁共振定量检测人类化 Duchenne 肌营养不良症小鼠模型骨骼肌脂质浸润。
NMR Biomed. 2023 Mar;36(3):e4869. doi: 10.1002/nbm.4869. Epub 2022 Nov 29.
6
The new challenge of "exercise + X″ therapy for Duchenne muscular dystrophy-Individualized identification of exercise tolerance and precise implementation of exercise intervention.杜氏肌营养不良症“运动 + X”疗法的新挑战——运动耐受性的个体化识别与运动干预的精准实施
Front Physiol. 2022 Aug 5;13:947749. doi: 10.3389/fphys.2022.947749. eCollection 2022.
7
Case Report: Advanced Skeletal Muscle Imaging in S-Adenosylhomocysteine Hydrolase Deficiency and Further Insight Into Muscle Pathology.病例报告：S-腺苷同型半胱氨酸水解酶缺乏症中的高级骨骼肌成像及对肌肉病理学的进一步洞察。
Front Pediatr. 2022 Apr 8;10:847445. doi: 10.3389/fped.2022.847445. eCollection 2022.
8
Effects of muscle damage on phosphorus magnetic resonance spectroscopy indices of energetic status and sarcolemma integrity in young mdx mice.肌肉损伤对年轻 mdx 小鼠能量状态和肌细胞膜完整性的磷磁共振波谱指标的影响。
NMR Biomed. 2022 Mar;35(3):e4659. doi: 10.1002/nbm.4659. Epub 2021 Nov 28.
9
Mouse Models of Muscle Fibrosis.肌肉纤维化的小鼠模型
Methods Mol Biol. 2021;2299:357-370. doi: 10.1007/978-1-0716-1382-5_24.
10
Beneficial Role of Exercise in the Modulation of Muscle Plastic Remodeling and Oxidative Stress.运动在调节肌肉可塑性重塑和氧化应激中的有益作用。
Antioxidants (Basel). 2021 Apr 3;10(4):558. doi: 10.3390/antiox10040558.
本文引用的文献
1
Widespread muscle expression of an AAV9 human mini-dystrophin vector after intravenous injection in neonatal dystrophin-deficient dogs.静脉注射携带人微小肌营养不良蛋白基因的 AAV9 载体后，在新生期肌营养不良症犬中广泛表达肌肉。
Mol Ther. 2010 Aug;18(8):1501-8. doi: 10.1038/mt.2010.94. Epub 2010 Jun 1.
2
T2 mapping in Duchenne muscular dystrophy: distribution of disease activity and correlation with clinical assessments.杜氏肌营养不良症中的 T2 映射：疾病活动的分布与临床评估的相关性。
Radiology. 2010 Jun;255(3):899-908. doi: 10.1148/radiol.10091547.
3
Prevalence of Duchenne/Becker muscular dystrophy among males aged 5-24 years - four states, 2007.2007年四个州5至24岁男性中杜氏/贝克型肌营养不良症的患病率
MMWR Morb Mortal Wkly Rep. 2009 Oct 16;58(40):1119-22.
4
Efficacy of systemic morpholino exon-skipping in Duchenne dystrophy dogs.全身性吗啉代外显子跳跃疗法对杜氏肌营养不良犬的疗效。
Ann Neurol. 2009 Jun;65(6):667-76. doi: 10.1002/ana.21627.
5
Preclinical drug trials in the mdx mouse: assessment of reliable and sensitive outcome measures.杜兴氏肌营养不良症小鼠的临床前药物试验：可靠且敏感的结果指标评估
Muscle Nerve. 2009 May;39(5):591-602. doi: 10.1002/mus.21211.
6
Mammalian animal models for Duchenne muscular dystrophy.杜氏肌营养不良症的哺乳动物动物模型。
Neuromuscul Disord. 2009 Apr;19(4):241-9. doi: 10.1016/j.nmd.2008.11.015. Epub 2009 Feb 12.
7
Validation of a numerical model of skeletal muscle compression with MR tagging: a contribution to pressure ulcer research.基于磁共振标记的骨骼肌压缩数值模型的验证：对压疮研究的贡献
J Biomech Eng. 2008 Dec;130(6):061015. doi: 10.1115/1.2987877.
8
Sarcolemma-localized nNOS is required to maintain activity after mild exercise.轻度运动后，维持活性需要肌膜定位的神经元型一氧化氮合酶。
Nature. 2008 Nov 27;456(7221):511-5. doi: 10.1038/nature07414. Epub 2008 Oct 26.
9
Towards developing standard operating procedures for pre-clinical testing in the mdx mouse model of Duchenne muscular dystrophy.致力于制定杜氏肌营养不良症mdx小鼠模型临床前测试的标准操作程序。
Neurobiol Dis. 2008 Jul;31(1):1-19. doi: 10.1016/j.nbd.2008.03.008. Epub 2008 Apr 9.
10
Comparative MRI analysis of T2 changes associated with single and repeated bouts of downhill running leading to eccentric-induced muscle damage.与单次及反复下坡跑导致离心性肌肉损伤相关的T2变化的对比MRI分析。
J Appl Physiol (1985). 2008 Jul;105(1):299-307. doi: 10.1152/japplphysiol.00738.2007. Epub 2008 May 1.
Zotero 插件