Department of Microbiology and Immunology, Division of Infectious Diseases, New York Medical College, Valhalla, New York 10595, USA.
J Clin Microbiol. 2011 Jun;49(6):2166-8. doi: 10.1128/JCM.00350-11. Epub 2011 Apr 13.
Approximately 45% of untreated United States patients with early Lyme disease associated with erythema migrans have a positive blood culture based on microscopic detection of Borrelia burgdorferi in Barbour-Stoenner-Kelly medium after 2 to 12 weeks of incubation. In this study we demonstrate that the yield of blood cultures can be significantly increased to 70.8% by the use of a combined culture-quantitative PCR technique and that among those patients found to have a positive blood culture, positivity was detected in over 90% within just 7 days of incubation. Patients with multiple erythema migrans were almost uniformly culture positive by this technique.
约 45%未经治疗的美国早期莱姆病（与游走性红斑有关）患者，在巴尔博-斯通纳-凯利培养基中孵育 2 至 12 周后，通过显微镜检测伯氏疏螺旋体，血液培养呈阳性。在这项研究中，我们证明通过联合培养定量 PCR 技术，血液培养的产量可以显著提高到 70.8%，并且在那些发现血液培养阳性的患者中，超过 90%的患者在孵育 7 天内即可检测到阳性。使用该技术，多发性游走性红斑患者几乎全部为培养阳性。
