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静脉注射抗 HER2 抗体偶联 CdSe/ZnS 量子点在 Wistar 大鼠体内的分布与毒性评价。

Bio-distribution and toxicity assessment of intravenously injected anti-HER2 antibody conjugated CdSe/ZnS quantum dots in Wistar rats.

机构信息

School of Environmental Sciences, Jawaharlal Nehru University, New Delhi, India.

出版信息

Int J Nanomedicine. 2011;6:463-75. doi: 10.2147/IJN.S15124. Epub 2011 Feb 25.

DOI:10.2147/IJN.S15124
PMID:21499435
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3075911/
Abstract

Anti-HER2 antibody conjugated with quantum dots (anti-HER2ab-QDs) is a very recent fluorescent nanoprobe for HER2+ve breast cancer imaging. In this study we investigated in-vivo toxicity of anti-HER2ab conjugated CdSe/ZnS QDs in Wistar rats. For toxicity evaluation of injected QDs sample, body weight, organ coefficient, complete blood count (CBC), biochemistry panel assay (AST, ALT, ALP, and GGTP), comet assay, reactive oxygen species, histology, and apoptosis were determined. Wistar rat (8-10 weeks old) were randomly divided into 4 treatment groups (n = 6). CBC and biochemistry panel assay showed nonsignificant changes in the anti-HER2ab-QDs treated group but these changes were significant (P < 0.05) in QDs treated group. No tissue damage, inflammation, lesions, and QDs deposition were found in histology and TEM images of the anti-HER2ab-QDs treated group. Apoptosis in liver and kidney was not found in the anti-HER2ab-QDs treated group. Animals treated with nonconjugated QDs showed comet formation and apoptosis. Cadmium deposition was confirmed in the QDs treated group compared with the anti-HER2ab-QDs treated group. The QDs concentration (500 nM) used for this study is suitable for in-vivo imaging. The combine data of this study support the biocompatibility of anti-HER2ab-QDs for breast cancer imaging, suggesting that the antibody coating assists in controlling any possible adverse effect of quantum dots.

摘要

抗 HER2 抗体偶联量子点（抗 HER2ab-QDs）是一种最近用于 HER2+阳性乳腺癌成像的荧光纳米探针。本研究考察了抗 HER2ab 偶联 CdSe/ZnS QDs 在 Wistar 大鼠体内的毒性。为了评估注射 QDs 样品的毒性，测定了体重、器官系数、全血细胞计数（CBC）、生物化学分析（AST、ALT、ALP 和 GGTP）、彗星试验、活性氧、组织学和细胞凋亡。Wistar 大鼠（8-10 周龄）随机分为 4 个治疗组（n = 6）。CBC 和生物化学分析显示抗 HER2ab-QDs 治疗组无显著变化，但 QDs 治疗组有显著变化（P < 0.05）。抗 HER2ab-QDs 治疗组的组织学和 TEM 图像未见组织损伤、炎症、病变和 QDs 沉积。抗 HER2ab-QDs 治疗组的肝、肾未见细胞凋亡。未偶联 QDs 治疗的动物表现出彗星形成和细胞凋亡。与抗 HER2ab-QDs 治疗组相比，QDs 治疗组证实有镉沉积。与体内成像相比，本研究中使用的 QDs 浓度（500 nM）更适合。本研究的综合数据支持抗 HER2ab-QDs 用于乳腺癌成像的生物相容性，表明抗体涂层有助于控制量子点的任何潜在不良反应。

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