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改良显色培养基 ChromID MRSA New 用于检测临床标本中耐甲氧西林金黄色葡萄球菌的性能评估。

Performance evaluation of a modified chromogenic medium, ChromID MRSA New, for the detection of methicillin-resistant Staphylococcus aureus from clinical specimens.

机构信息

Department of Clinical Chemistry, Microbiology and Immunology, Ghent University Hospital, Ghent, Belgium.

出版信息

Eur J Clin Microbiol Infect Dis. 2011 Dec;30(12):1595-8. doi: 10.1007/s10096-011-1265-3. Epub 2011 Apr 16.

Abstract

A novel chromogenic medium for the detection of methicillin-resistant Staphylococcus aureus (MRSA), ChromID MRSA New, was evaluated and compared with the original ChromID MRSA agar, using 355 consecutive screening specimens from nose (120), throat (121) and perineum (114). The specimens were collected with an E-swab and inoculated within 24 hours onto both ChromID MRSA New and on ChromID MRSA. ChromID MRSA New was more sensitive than ChromID MRSA in detecting MRSA after 24 hours of incubation (94.3% versus 81.4%; p < 0.05). With the ChromID MRSA New, processing time is reduced from 48 h to 24 h and confirmation of the resistance to methicillin is redundant.

摘要

一种新型显色培养基用于检测耐甲氧西林金黄色葡萄球菌(MRSA),称为 ChromID MRSA New,与原始的 ChromID MRSA 琼脂进行了评估和比较,使用了 355 例连续的鼻腔(120 例)、咽喉(121 例)和会阴(114 例)筛查标本。这些标本用 E-拭子采集,并在 24 小时内接种到 ChromID MRSA New 和 ChromID MRSA 上。孵育 24 小时后,ChromID MRSA New 比 ChromID MRSA 检测 MRSA 更敏感(94.3%比 81.4%;p<0.05)。使用 ChromID MRSA New,处理时间从 48 小时缩短到 24 小时,并且对耐甲氧西林的确认是多余的。

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