Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, North 18, West 9, Kita-ku, Sapporo, 060-0818, Japan.
Arch Virol. 2011 Aug;156(8):1379-85. doi: 10.1007/s00705-011-1000-5. Epub 2011 Apr 20.
A/equine/Kanazawa/1/2007 (H3N8), A/equine/Hokkaido/I828/2008 (H3N8) and A/equine/Mongolia/1/2008 (H3N8) were isolated from infected horses. A/equine/Yokohama/aq19/2009 (H3N8) and A/equine/Yokohama/aq13/2010 (H3N8) were isolated from horses imported from Canada and Belgium examined at the Animal Quarantine Service in Yokohama, Japan. In the present study, these five isolates were genetically and antigenically analyzed. Phylogenetic analysis of hemagglutinin (HA) and neuraminidase (NA) genes showed that three isolates from horses in Japan and imported from Canada belonged to the same branch, clade 1 of the Florida sublineage, while the isolates from horses in Mongolia and imported from Belgium belonged to another branch, clade 2 of the Florida sublineage. Reactivity patterns of a panel of monoclonal antibodies to the HA of A/equine/Kanazawa/1/2007 (H3N8) with the five isolates indicate that the HAs of these viruses were antigenically similar to each other and to the reference strains A/equine/La Plata/1/1993 (H3N8) and A/equine/Avesta/1/1993 (H3N8). The present findings indicate that extensive antigenic variation has not accumulated among H3N8 influenza viruses in horses.
从感染马中分离到 A/马/金泽/1/2007（H3N8）、A/马/北海道/I828/2008（H3N8）和 A/马/蒙古/1/2008（H3N8）。从日本横滨动物检疫所检查的从加拿大和比利时进口的马中分离到 A/马/横滨/aq19/2009（H3N8）和 A/马/横滨/aq13/2010（H3N8）。在本研究中，对这五个分离株进行了遗传和抗原分析。血凝素（HA）和神经氨酸酶（NA）基因的系统发育分析表明，来自日本和从加拿大进口的三株分离株属于同一分支，即佛罗里达亚系的 1 分支，而来自蒙古和从比利时进口的分离株属于另一分支，即佛罗里达亚系的 2 分支。用一组针对 A/马/金泽/1/2007（H3N8）HA 的单克隆抗体对这五个分离株的反应模式表明，这些病毒的 HAs 在抗原上彼此相似，并且与参考株 A/马/拉普拉塔/1/1993（H3N8）和 A/马/阿韦斯塔/1/1993（H3N8）相似。目前的发现表明，马源 H3N8 流感病毒之间没有积累广泛的抗原变异。
