Wang Li, Jing Ming-xin, Zhi Jian-mei, Lu Jun, Wang Chao-yang, Liu Qing-guo
TCM International Cooperation Center of China, Beijing 100101, China.
Zhongguo Zhen Jiu. 2011 Apr;31(4):337-41.
To explore the different effects of reinforcing and reducing methods by twirling and rotating the needle on stress-induced hypertension and its mechanism.
Sixty male Wistar rats were randomly divided into a nomal group (group A), a model group (group B), an acupuncture control group (group C), an acupuncture with reinforcing method group (group D)and an acupuncture with reducing method group (group E), 12 rats in each group. The model of stress-induced hypertension was established by foot-shock and noise stimulation in the other groups except group A. Group C was treated by acupuncture at left "Taichong" (LR 3) with no manipulation, group D with twirling reinforcement method and group E with twirling reducing method at the same acupoint, respectively, and group A and B with no acupuncture treatment. The contents of calcitonin gene-related peptide (CGRP) and nitrogen monoxidum (NO) in blood plasma and hypothalamus after 7 days were observed.
After acupuncture treatment, the contents of CGRP in blood plasma and hypothalamus were (375.9 +/- 36.5) pg/mL and (213.6 +/- 50.1) pg/mg in group A, which were higher than (261.0 +/- 20.1) pg/mL and (156.0 +/- 21.8) pg/mg in group B , and (255.3 +/- 33.6) pg/mL and (154.3 +/- 47.3) pg/mg in group C (all P < 0.05), and (465.9 +/- 53.2) pg/mL and (250.74 +/- 47.7) pg/mg in group E higher than those in group C and (283.3 +/- 29.8) pg/mL and (164.6 +/- 47.4) pg/mg in group D (all P < 0.05), and there was no significant difference among group B, C and D (all P > 0.05). After acupuncture treatment, the contents of NO in blood plasma and hypothalamus were (43.7 +/- 5.5) micromol/L and (23.3 +/- 2.9) micromol/g in group B, (46.5 +/- 6.4) micromol/L and (23.1 +/- 3.3) micromol/g in group C, (45.8 +/- 6.7) micromol/L and (25.0 +/- 2.7) micromol/g in group D, which were all lower than (64.5 +/- 9.9) micromol/L and (30.7 +/- 1.6) micromol/g in group A (all P < 0.05), (55.9 +/- 4.6) micromol/L and (30.4 +/- 2.1) micromol/g in group E higher than those in group C and group D (all P < 0.05), and there was no significant difference among group B, C and D either (all P > 0.05).
Acupuncture with twirling reducing method can increase the contents of CGRP and NO in rats with stress-induced hypertension, thereby causing a fall in blood pressure, however, no significant influence with twirling reinforcing method. Reinforcing or reducing method by twirling and rotating the needle has different efficacy.
探讨捻转补泻法对应激性高血压的不同影响及其机制。
将60只雄性Wistar大鼠随机分为正常组（A组）、模型组（B组）、针刺对照组（C组）、捻转补法组（D组）和捻转泻法组（E组），每组12只。除A组外，其余各组采用足底电击和噪声刺激建立应激性高血压模型。C组针刺左侧“太冲”（LR 3），不提插捻转；D组和E组分别采用捻转补法和捻转泻法针刺同一穴位；A组和B组不针刺。观察7天后血浆及下丘脑降钙素基因相关肽（CGRP）和一氧化氮（NO）含量。
针刺治疗后，A组血浆及下丘脑CGRP含量分别为（375.9±36.5）pg/mL和（213.6±50.1）pg/mg，高于B组的（261.0±20.1）pg/mL和（156.0±21.8）pg/mg，C组的（255.3±33.6）pg/mL和（154.3±47.3）pg/mg（均P<0.05）；E组血浆及下丘脑CGRP含量分别为（465.9±53.2）pg/mL和（250.74±47.7）pg/mg，高于C组及D组的（283.3±29.8）pg/mL和（164.6±47.4）pg/mg（均P<0.05）；B、C、D组间比较差异无统计学意义（均P>0.05）。针刺治疗后，B组血浆及下丘脑NO含量分别为（43.7±5.5）μmol/L和（23.3±2.9）μmol/g，C组为（46.5±6.4）μmol/L和（23.1±3.3）μmol/g，D组为（45.8±6.7）μmol/L和（25.0±2.7）μmol/g，均低于A组的（64.5±9.9）μmol/L和（30.7±1.6）μmol/g（均P<0.05）；E组血浆及下丘脑NO含量分别为（55.9±4.6）μmol/L和（30.4±2.1）μmol/g，高于C组和D组（均P<0.05）；B、C、D组间比较差异无统计学意义（均P>0.05）。
捻转泻法针刺可提高应激性高血压大鼠CGRP和NO含量，从而使血压下降，而捻转补法无明显影响。捻转补泻法具有不同的效应。
