Colin Ratledge Center for Microbial Lipids, School of Agriculture Engineering and Food Sciences, Shandong University of Technology, Shandong Zibo 255000, China.
Normal College, Jishou University, Jishou, 416000 Hunan, China.
Biomed Res Int. 2021 Sep 21;2021:5514745. doi: 10.1155/2021/5514745. eCollection 2021.
Lignocellulosic waste is the most abundant biorenewable biomass on earth, and its hydrolysis releases highly valued reducing sugars. However, the presence of lignin in the biopolymeric structure makes it highly resistant to solubilization thereby hindering the hydrolysis of cellulose and hemicellulose. Microorganisms are known for their potential complex enzymes that play a dominant role in lignocellulose conversion. Therefore, the current study was designed to isolate and screen potential microorganisms for their selective delignification ability for the pretreatment of lignocellulosic biomass. An extensive isolation and screening procedure yielded 36 desired isolates (22 bacteria, 7 basidiomycete fungi, and 7 filamentous fungi). Submerged cultivation of these desired microorganisms revealed 4 bacteria and 10 fungi with potent lignocellulolytic enzyme activities. The potent isolates were identified as , , , , and spp. confirmed by morphological and molecular identification. The efficiency of these strains was determined through enzyme activities, and the degraded substrates were analyzed through scanning electron microscopy (SEM) and X-ray diffraction (XRD). Among all isolated microbes, spp. were found to have high laccase activity. The cellulose-decomposing and selective delignification strains were subjected to solid-state fermentation (SSF). SSF of field waste corn stalks as a single-carbon source provides spp. better condition for the secretion of ligninolytic enzymes. These isolated ligninolytic enzymes producing microorganisms may be used for the effective pretreatment of lignocellulosic agricultural wastes for the production of high value-added natural products by fermentation.
木质纤维素废物是地球上最丰富的生物可再生生物质，其水解释放出高价值的还原糖。然而，木质素存在于生物聚合物结构中，使其高度抵抗溶解，从而阻碍纤维素和半纤维素的水解。微生物以其潜在的复杂酶而闻名，这些酶在木质纤维素转化中发挥着主导作用。因此，本研究旨在分离和筛选具有选择性脱木质素能力的潜在微生物，用于木质纤维素生物质的预处理。广泛的分离和筛选程序产生了 36 个所需的分离物（22 个细菌、7 个担子菌真菌和 7 个丝状真菌）。这些所需微生物的深层培养揭示了 4 种细菌和 10 种真菌具有很强的木质纤维素酶活性。通过形态学和分子鉴定证实，这些强分离株为 、 、 、 和 spp。通过酶活性测定了这些菌株的效率，并通过扫描电子显微镜 (SEM) 和 X 射线衍射 (XRD) 分析了降解的底物。在所有分离的微生物中， spp. 被发现具有高漆酶活性。纤维素分解和选择性脱木质素菌株进行固态发酵（SSF）。作为单一碳源的田间废玉米秸秆的 SSF 为 spp. 分泌木质素酶提供了更好的条件。这些分离出的产木质素酶微生物可用于有效预处理木质纤维素农业废物，通过发酵生产高附加值天然产物。
