Department of Gastroenterology and Hepatology, Erasmus MC, University Medical Center, Rotterdam, The Netherlands.
Mol Immunol. 2011 Jul;48(12-13):1505-11. doi: 10.1016/j.molimm.2011.04.008. Epub 2011 Apr 30.
Little is known about the frequency and function of CD16(+)CD14(-) monocytes from chronic HCV patients. We observed that the absolute numbers and ratio of CD16(+)CD14(-) to CD14(+)CD16(-) monocytes were similar between chronic HCV patients and healthy individuals. Functionally, we found that CD16(+)CD14(-) monocytes are more responsive to TLR8-ligation and only weakly responsive to LPS stimulation in producing TNF as compared to CD14(+)CD16(-) monocytes. We found no overt impairment of the function of CD16(+)CD14(-) monocytes from patients, except for an augmented induction of MIP-1β-producing CD16(+)CD14(-) monocytes upon TLR4-ligation. However, the increased frequency of MIP-1β-producing CD16(+)CD14(-) monocytes was not associated with viral load, ALT or fibrosis level. Our findings indicate that, different from other infectious diseases, the frequency and function of CD16(+)CD14(-) monocytes are only minimally altered as a consequence of the persistent state of HCV infections, and our findings therefore do not suggest a role for CD16(+)CD14(-) monocytes in HCV pathogenesis.
人们对慢性 HCV 患者中 CD16(+)CD14(-)单核细胞的频率和功能知之甚少。我们观察到，慢性 HCV 患者和健康个体之间 CD16(+)CD14(-)单核细胞的绝对数量和 CD14(+)CD16(-)与 CD16(+)CD14(-)单核细胞的比例相似。在功能上，我们发现与 CD14(+)CD16(-)单核细胞相比，CD16(+)CD14(-)单核细胞对 TLR8 配体的反应更敏感，而对 LPS 刺激产生 TNF 的反应较弱。我们没有发现患者的 CD16(+)CD14(-)单核细胞功能明显受损，除了 TLR4 配体诱导后产生 MIP-1β的 CD16(+)CD14(-)单核细胞的诱导增加。然而，产生 MIP-1β的 CD16(+)CD14(-)单核细胞的频率增加与病毒载量、ALT 或纤维化水平无关。我们的研究结果表明，与其他传染病不同，由于 HCV 感染的持续状态，CD16(+)CD14(-)单核细胞的频率和功能仅发生微小改变，因此我们的研究结果并不表明 CD16(+)CD14(-)单核细胞在 HCV 发病机制中起作用。
