PtrA 是一种参与铜耐受的铜绿假单胞菌周质蛋白。
PtrA is a periplasmic protein involved in Cu tolerance in Pseudomonas aeruginosa.
机构信息
Bacterial Pathogenesis and Cellular Responses, iRTSV, CEA-Grenoble, Grenoble cedex 09, France.
出版信息
J Bacteriol. 2011 Jul;193(13):3376-8. doi: 10.1128/JB.00159-11. Epub 2011 Apr 29.
Abstract
In this work, we demonstrate that PtrA (U. H. Ha et al., Mol. Microbiol. 54:307-320, 2004) is a periplasmic protein, specifically synthesized in the presence of copper, that is involved in the tolerance of Pseudomonas aeruginosa to copper. Our biochemical and genetic analyses challenge its role in transcriptional inhibition of the type III secretion system.
摘要
在这项工作中，我们证明了 PtrA（U. H. Ha 等人，Mol. Microbiol. 54:307-320, 2004）是一种周质蛋白，在铜存在的情况下特异性合成，参与铜对铜绿假单胞菌的耐受性。我们的生化和遗传分析挑战了它在 III 型分泌系统转录抑制中的作用。
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J Bacteriol. 2010 Mar;192(6):1479-86. doi: 10.1128/JB.01457-09. Epub 2009 Dec 11.
2
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3
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8
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