完整和缺陷型SV40基因组在小鼠-人类体细胞杂种中的串联整合。

Tandem integration of complete and defective SV40 genomes in mouse-human somatic cell hybrids.

作者信息

Campo M S, Cameron I R, Rogers M E

出版信息

Cell. 1978 Dec;15(4):1411-26. doi: 10.1016/0092-8674(78)90065-x.

Abstract

We have analyzed the arrangement of SV40 DNA sequences integrated in human chromosome 7 in two lines of mouse-human somatic cell hybrids: one containing only one human chromosome 7 per cell and the other containing an average of about three. We found that the integration site differs in both the viral and host sequences in the two clones. However, the sites of integration into the several copies of human chromosome 7 of one clone are identical. Each chromosome 7 in both clones carries approximately six viral genomes tandemly linked. Some of these genomes lack about 20% of the DNA from the late region, including the Eco RI site.

摘要

我们分析了整合在两条小鼠 - 人类体细胞杂种系人染色体7中的SV40 DNA序列的排列情况：一个杂种系每个细胞仅含一条人染色体7，另一个杂种系平均每个细胞含约三条人染色体7。我们发现，在这两个克隆中，病毒和宿主序列的整合位点均不同。然而，一个克隆中人染色体7的几个拷贝的整合位点是相同的。两个克隆中的每条染色体7都串联连接着大约六个病毒基因组。其中一些基因组缺少约20%的来自晚期区域的DNA，包括Eco RI位点。