接种JC病毒的夜猴脑肿瘤含有JC病毒基因组。
Brain tumors of owl monkeys inoculated with JC virus contain the JC virus genome.
作者信息
Miller N R, McKeever P E, London W, Padgett B L, Walker D L, Wallen W C
出版信息
J Virol. 1984 Mar;49(3):848-56. doi: 10.1128/JVI.49.3.848-856.1984.
The DNA from astrocytomas that developed in adult owl monkeys 16 to 36 months after intracranial inoculation with JC virus (JCV) was examined for the presence of the JCV genome by hybridization to cloned JCV DNA. The JCV genome was found to be integrated into the cellular DNA in all tumors examined. There was no JCV DNA in normal, uninvolved brain tissue from the same animals. Integration of the genome occurred at a limited number of sites in the cellular DNAs, indicating a clonal origin for the tumors, but none of the tumors had integration sites in common. In all but one of the tumors, there was tandem, head-to-tail integration of two or more copies of the JC genome. In a tumor which had only one integration site and could be analyzed more extensively, there appeared to be a complete copy of the JCV genome present, although deletions of small portions of the genome would not have been detected.
对成年猫头鹰猴在颅内接种JC病毒（JCV）后16至36个月发生的星形细胞瘤的DNA进行检测，通过与克隆的JCV DNA杂交来检查JCV基因组的存在情况。在所检查的所有肿瘤中，发现JCV基因组整合到了细胞DNA中。来自同一动物的正常、未受累脑组织中没有JCV DNA。基因组整合发生在细胞DNA的有限位点，表明肿瘤起源于克隆，但没有一个肿瘤有共同的整合位点。除了一个肿瘤外，所有肿瘤中都有两个或更多JC基因组拷贝的串联、头对头整合。在一个只有一个整合位点且能进行更广泛分析的肿瘤中，似乎存在JCV基因组的完整拷贝，不过基因组小部分的缺失是检测不到的。
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