Kucherlapati R, Hwang S P, Shimizu N, McDougall J K, Botchan M R
Proc Natl Acad Sci U S A. 1978 Sep;75(9):4460-4. doi: 10.1073/pnas.75.9.4460.
Somatic cell hybrids derived from fusion of GM637, a human cell line transformed by simian virus 40, and mouse B82 cells were examined for simian virus 40 T antigen, V antigen, and viral DNA. All hybrid cell lines that contained viral DNA were T-antigen positive. Cells that did not have viral DNA were T-antigen negative. We determined that there is a single viral insertion in these hybrid cells. Correlation of T-antigen expression and viral DNA with the partial complements of the human genome retained in the hybrids shwed that the inserted viral genome is in human chromosome 8. The integrated viral DNA is stable; free viral DNA found in GM637 does not insert at other potential sites in the human genome.
对由猿猴病毒40转化的人细胞系GM637与小鼠B82细胞融合产生的体细胞杂种进行了猿猴病毒40 T抗原、V抗原和病毒DNA检测。所有含有病毒DNA的杂种细胞系T抗原均呈阳性。没有病毒DNA的细胞T抗原呈阴性。我们确定这些杂种细胞中有单个病毒插入。T抗原表达和病毒DNA与杂种中保留的人类基因组部分互补体的相关性表明，插入的病毒基因组位于人类8号染色体上。整合的病毒DNA是稳定的；GM637中发现的游离病毒DNA不会插入人类基因组的其他潜在位点。
