使用新型荧光探针评估心血管纤维化。
Assessment of cardiovascular fibrosis using novel fluorescent probes.
机构信息
Cardiovascular Research Center, Mount Sinai School of Medicine, New York, New York, United States of America.
出版信息
PLoS One. 2011 Apr 20;6(4):e19097. doi: 10.1371/journal.pone.0019097.
Abstract
Cardiovascular fibrosis resulted from pressure overload or ischemia could alter myocardial stiffness and lead to ventricular dysfunction. Fluorescently labeled collagen-binding protein CNA 35, derived from the surface component of Staphylococcus aureus, and a novel synthetic biphenylalanine containing peptide are applied to stain fibrosis associated collagen and myocytes, respectively. Detailed pathological characteristics of cardiovascular fibrosis could be identified clearly in 2 hours. This staining pair requires only simple staining and brief washing, generating less than 10 ml of waste. The image information collected by this novel fluorescent staining pair is compatible with it collected by the traditional Masson's Trichrome and Picrosirius Red staining which are widely used to stain cardiovascular fibrosis and isolated cells.
摘要
心血管纤维化是由压力超负荷或缺血引起的，可改变心肌硬度，导致心室功能障碍。荧光标记的胶原结合蛋白 CNA35 来源于金黄色葡萄球菌表面成分，一种新型合成的含二苯丙氨酸的肽分别用于染色纤维化相关的胶原和心肌细胞。在 2 小时内可以清楚地识别心血管纤维化的详细病理特征。这种染色对仅需要简单染色和短暂洗涤，产生的废物少于 10 毫升。这种新型荧光染色对收集的图像信息与广泛用于染色心血管纤维化和分离细胞的传统 Masson 三色和苦味酸红染色收集的信息兼容。
相似文献
1
Assessment of cardiovascular fibrosis using novel fluorescent probes.使用新型荧光探针评估心血管纤维化。
PLoS One. 2011 Apr 20;6(4):e19097. doi: 10.1371/journal.pone.0019097.
2
Comparative measurement of collagen bundle orientation by Fourier analysis and semiquantitative evaluation: reliability and agreement in Masson's trichrome, Picrosirius red and confocal microscopy techniques.通过傅里叶分析和半定量评估对胶原束取向进行比较测量：Masson三色染色法、天狼星红苦味酸染色法和共聚焦显微镜技术的可靠性及一致性
J Microsc. 2017 Aug;267(2):130-142. doi: 10.1111/jmi.12553. Epub 2017 Mar 20.
3
Cholecystokinin octapeptide reduces myocardial fibrosis and improves cardiac remodeling in post myocardial infarction rats.八肽胆囊收缩素可减少心肌梗死后大鼠的心肌纤维化，改善心脏重构。
Int J Biochem Cell Biol. 2020 Aug;125:105793. doi: 10.1016/j.biocel.2020.105793. Epub 2020 Jun 15.
4
Qishen granule attenuates cardiac fibrosis by regulating TGF-β /Smad3 and GSK-3β pathway.芪参颗粒通过调控 TGF-β/Smad3 和 GSK-3β 通路减轻心肌纤维化。
Phytomedicine. 2019 Sep;62:152949. doi: 10.1016/j.phymed.2019.152949. Epub 2019 May 8.
5
Analysis of Generalized Fibrosis in Mouse Tissue Sections with Masson's Trichrome Staining.用马松三色染色法分析小鼠组织切片中的广泛性纤维化
Bio Protoc. 2020 May 20;10(10):e3629. doi: 10.21769/BioProtoc.3629.
6
Utility of desmin and a Masson's trichrome method to detect early acute myocardial infarction in autopsy tissues.结蛋白和马松三色染色法在尸检组织中检测早期急性心肌梗死的效用。
Int J Clin Exp Pathol. 2009 Sep 20;3(1):98-105.
7
Renal Denervation Effects on Myocardial Fibrosis and Ventricular Arrhythmias in Rats with Ischemic Cardiomyopathy.肾去神经支配对缺血性心肌病大鼠心肌纤维化和室性心律失常的影响
Cell Physiol Biochem. 2018;46(6):2471-2479. doi: 10.1159/000489653. Epub 2018 May 5.
8
Pigment epithelium-derived factor attenuates myocardial fibrosis via inhibiting Endothelial-to-Mesenchymal Transition in rats with acute myocardial infarction.色素上皮衍生因子通过抑制急性心肌梗死后大鼠的内皮-间质转化来减轻心肌纤维化。
Sci Rep. 2017 Feb 7;7:41932. doi: 10.1038/srep41932.
9
Staining efficacy assessment of a differential routine and special stains for pathological stromal calcifications in maxillofacial lesions.评估口腔颌面部病变中不同常规和特殊染色法对病理性间质钙化的染色效果。
J Histotechnol. 2020 Sep;43(3):118-124. doi: 10.1080/01478885.2020.1739192. Epub 2020 Mar 10.
10
The use of Masson's trichrome staining, second harmonic imaging and two-photon excited fluorescence of collagen in distinguishing intestinal tuberculosis from Crohn's disease.利用马松三色染色、二次谐波成像和胶原蛋白的双光子激发荧光鉴别肠结核和克罗恩病。
Colorectal Dis. 2016 Dec;18(12):1172-1178. doi: 10.1111/codi.13400.
引用本文的文献
1
Exploring the relationship between epigenetic DNA methylation and cardiac fibrosis through Raman microspectroscopy.通过拉曼微光谱技术探索 DNA 甲基化的表观遗传学与心脏纤维化之间的关系。
Am J Physiol Cell Physiol. 2023 Jul 1;325(1):C332-C343. doi: 10.1152/ajpcell.00209.2023. Epub 2023 Jun 19.
2
It's clearly the heart! Optical transparency, cardiac tissue imaging, and computer modelling.这显然是心脏！光学透明性、心脏组织成像和计算机建模。
Prog Biophys Mol Biol. 2022 Jan;168:18-32. doi: 10.1016/j.pbiomolbio.2021.06.005. Epub 2021 Jun 11.
3
Proteomic and Structural Manifestations of Cardiomyopathy in Rat Models of Obesity and Weight Loss.肥胖和减肥大鼠模型中心肌病的蛋白质组学和结构表现。
Front Endocrinol (Lausanne). 2021 Feb 24;12:568197. doi: 10.3389/fendo.2021.568197. eCollection 2021.
4
Intralacrimal Sustained Delivery of Rapamycin Shows Therapeutic Effects without Systemic Toxicity in a Mouse Model of Autoimmune Dacryoadenitis Characteristic of Sjögren's Syndrome.在特发性干燥综合征特征性自身免疫性泪腺炎的小鼠模型中，局部持续递送雷帕霉素显示出治疗效果而无全身毒性。
Biomacromolecules. 2021 Mar 8;22(3):1102-1114. doi: 10.1021/acs.biomac.0c01468. Epub 2020 Dec 27.
5
A collagen-binding protein enables molecular imaging of kidney fibrosis in vivo.一种胶原蛋白结合蛋白能够在体内对肾纤维化进行分子成像。
Kidney Int. 2020 Mar;97(3):609-614. doi: 10.1016/j.kint.2019.08.029. Epub 2019 Sep 18.
6
Animal model of left atrial thrombus in congestive heart failure in rats.大鼠充血性心力衰竭左心房血栓动物模型。
Am J Physiol Heart Circ Physiol. 2019 Jul 1;317(1):H63-H72. doi: 10.1152/ajpheart.00086.2019. Epub 2019 May 10.
7
Emerging imaging targets for infiltrative cardiomyopathy: Inflammation and fibrosis.浸润性心肌病的新兴成像靶点：炎症与纤维化。
J Nucl Cardiol. 2019 Feb;26(1):208-216. doi: 10.1007/s12350-018-1356-y. Epub 2018 Jul 2.
8
Pharmacokinetics and microbiodistribution of 64Cu-labeled collagen-binding peptides in chronic myocardial infarction.64Cu标记的胶原结合肽在慢性心肌梗死中的药代动力学和微生物分布
Nucl Med Commun. 2016 Dec;37(12):1306-1317. doi: 10.1097/MNM.0000000000000590.
9
Abnormalities of capillary microarchitecture in a rat model of coronary ischemic congestive heart failure.冠状动脉缺血性充血性心力衰竭大鼠模型中毛细血管微结构的异常
Am J Physiol Heart Circ Physiol. 2015 Apr 15;308(8):H830-40. doi: 10.1152/ajpheart.00583.2014. Epub 2015 Feb 6.
10
Colorful protein-based fluorescent probes for collagen imaging.用于胶原蛋白成像的基于蛋白质的彩色荧光探针。
PLoS One. 2014 Dec 9;9(12):e114983. doi: 10.1371/journal.pone.0114983. eCollection 2014.
本文引用的文献
1
The opposing effects of CCN2 and CCN5 on the development of cardiac hypertrophy and fibrosis.CCN2 和 CCN5 对心肌肥厚和纤维化发展的拮抗作用。
J Mol Cell Cardiol. 2010 Aug;49(2):294-303. doi: 10.1016/j.yjmcc.2010.04.010. Epub 2010 Apr 27.
2
Effects of CXCR4 gene transfer on cardiac function after ischemia-reperfusion injury.CXCR4 基因转染对缺血再灌注损伤后心功能的影响。
Am J Pathol. 2010 Apr;176(4):1705-15. doi: 10.2353/ajpath.2010.090451. Epub 2010 Feb 4.
3
High-affinity peptide-based collagen targeting using synthetic phage mimics: from phage display to dendrimer display.使用合成噬菌体模拟物进行基于高亲和力肽的胶原蛋白靶向：从噬菌体展示到树枝状聚合物展示。
J Am Chem Soc. 2009 Aug 26;131(33):11683-5. doi: 10.1021/ja902285m.
4
Cardiac fibroblasts: at the heart of myocardial remodeling.心脏成纤维细胞：心肌重塑的核心
Pharmacol Ther. 2009 Aug;123(2):255-78. doi: 10.1016/j.pharmthera.2009.05.002. Epub 2009 May 19.
5
Long-term cardiac-targeted RNA interference for the treatment of heart failure restores cardiac function and reduces pathological hypertrophy.长期心脏靶向RNA干扰治疗心力衰竭可恢复心脏功能并减轻病理性肥大。
Circulation. 2009 Mar 10;119(9):1241-52. doi: 10.1161/CIRCULATIONAHA.108.783852. Epub 2009 Feb 23.
6
Morphology, binding behavior and MR-properties of paramagnetic collagen-binding liposomes.
Contrast Media Mol Imaging. 2009 Mar-Apr;4(2):81-8. doi: 10.1002/cmmi.266.
7
8
Periostin induces proliferation of differentiated cardiomyocytes and promotes cardiac repair.骨膜蛋白可诱导分化的心肌细胞增殖并促进心脏修复。
Nat Med. 2007 Aug;13(8):962-9. doi: 10.1038/nm1619. Epub 2007 Jul 15.
9
High resolution imaging of collagen organisation and synthesis using a versatile collagen specific probe.使用通用的胶原蛋白特异性探针进行胶原蛋白组织和合成的高分辨率成像。
J Struct Biol. 2007 Sep;159(3):392-9. doi: 10.1016/j.jsb.2007.04.008. Epub 2007 May 3.
10
The Enterococcus faecalis MSCRAMM ACE binds its ligand by the Collagen Hug model.粪肠球菌MSCRAMM ACE通过胶原拥抱模型结合其配体。
J Biol Chem. 2007 Jul 6;282(27):19629-37. doi: 10.1074/jbc.M611137200. Epub 2007 Mar 28.
Zotero 插件