Allergy and Clinical Immunology Unit, Sourasky Medical Center, Allergy and Immunology, Tel-Aviv, Israel.
Immunol Invest. 2011;40(6):657-66. doi: 10.3109/08820139.2011.575426. Epub 2011 May 4.
Adenoid hypertrophy is the most common cause of upper airway obstruction and sleep-disordered breathing in children, yet its pathogenesis remains unclear. The identification of the novel helper T cell subsets, Th17 cells and regulatory T cells (Tregs) could provide new insight into our understanding of the mechanisms involved in the development of this condition. The purpose of this study is to evaluate the adenoidal lymphocyte subsets to describe the percentage of various lymphocyte subsets in hypertrophied adenoids and correlate them with symptom severity. Twenty consecutive children undergoing adenoidectomy were included, and lymphocytes were isolated from their adenoids. T cell subpopulations were detected by flow cytometry using a fluoresceinated monoclonal antibody directed against a number of cell markers (CD4+, CD8+, CD25+, FOXP3 IL17+, and others). We found a significant negative linear correlation between the Th17/Treg ratio and the patients' clinical scores (R = -0.71 p < 0.005). The correlation was independent of age and gender. Decreased ratios of Th17/Treg subpopulations may play a role in the pathogenesis of adenoid hypertrophy.
腺样体肥大是儿童上呼吸道梗阻和睡眠呼吸紊乱最常见的原因，但发病机制尚不清楚。新型辅助性 T 细胞亚群 Th17 细胞和调节性 T 细胞（Tregs）的发现，为我们理解这种疾病的发展机制提供了新的认识。本研究旨在评估腺样体的淋巴细胞亚群，描述肥大腺样体中各种淋巴细胞亚群的百分比，并将其与症状严重程度相关联。连续纳入 20 例行腺样体切除术的儿童，并从其腺样体中分离出淋巴细胞。通过流式细胞术使用针对多种细胞标志物（CD4+、CD8+、CD25+、FOXP3、IL17+ 等）的荧光单克隆抗体来检测 T 细胞亚群。我们发现 Th17/Treg 比值与患者的临床评分之间存在显著的负线性相关（R = -0.71，p < 0.005）。这种相关性与年龄和性别无关。Th17/Treg 亚群比值的降低可能在腺样体肥大的发病机制中起作用。
