Soudani Nejla, Troudi Afef, Bouaziz Hanen, Boudawara Tahia, Zeghal Najiba
Animal Physiology Laboratory, Sfax Faculty of Sciences, University of Sfax, Tunisia.
Toxicol Ind Health. 2011 Sep;27(8):724-34. doi: 10.1177/0748233710395992. Epub 2011 May 4.
Exposure to hexavalent chromium Cr(VI) compounds is of concern in many Cr-related industries and their surrounding environments. K(2)Cr(2)O(7) is widely recognized as an animal and human carcinogen, mutagen, and teratogen. The present study investigated the bone maturity of suckling rats whose mothers were treated with K(2)Cr(2)O(7). Experiments were carried out on female Wistar rats given 700 ppm of K(2)Cr(2)O(7) in their drinking water from the 14th day of pregnancy until day 14 after delivery. Exposing dams to K(2)Cr(2)O(7) caused disorders in the bone of their progeny. As corollary to this, malondialdehyde levels increased, while glutathione, a non-protein thiol and vitamin C decreased. Alteration of the antioxidant system in the treated group was also confirmed by the significant decline of superoxide dismutase, catalase, and glutathione peroxidase activities. Furthermore, K(2)Cr(2)O(7) induced changes in bone mineralization, especially calcium and phosphorus levels, which decreased. Whereas, in plasma and urine, they increased and decreased inversely. These results suggest that K(2)Cr(2)O(7) accelerated bone resorption activity. In fact, in treated pups, total tartrate-resistant acid phosphatase, which reflected bone resorption, was enhanced while total alkaline phosphatase, which reflected bone formation, was reduced. The impairment of bone function was corresponded histologically.
在许多与铬相关的行业及其周边环境中，六价铬（Cr(VI)）化合物的暴露备受关注。重铬酸钾（K₂Cr₂O₇）被广泛认为是一种动物和人类致癌物、诱变剂和致畸剂。本研究调查了其母亲接受重铬酸钾处理的哺乳期大鼠的骨骼成熟情况。实验在怀孕第14天至产后第14天期间，给雌性Wistar大鼠饮用含700 ppm重铬酸钾的水。让母鼠接触重铬酸钾会导致其后代骨骼出现紊乱。与此相关的是，丙二醛水平升高，而非蛋白质硫醇谷胱甘肽和维生素C水平降低。处理组抗氧化系统的改变也通过超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶活性的显著下降得到证实。此外，重铬酸钾会引起骨矿化变化，尤其是钙和磷水平降低。而在血浆和尿液中，它们则呈相反变化，即血浆中升高而尿液中降低。这些结果表明重铬酸钾加速了骨吸收活动。事实上，在处理后的幼鼠中，反映骨吸收的抗酒石酸酸性磷酸酶总量增加，而反映骨形成的碱性磷酸酶总量减少。骨功能的损害在组织学上也得到了证实。
