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利用添加 EDTA 的美罗培南纸片对肠杆菌属金属β-内酰胺酶进行灵敏且特异的表型检测。

Sensitive and specific phenotypic assay for metallo-beta-lactamase detection in Enterobacteria by use of a moxalactam disk supplemented with EDTA.

机构信息

Service de Bactériologie, Centre Hospitalo-Universitaire d'Amiens, Hôpital Nord, 80054 Amiens, France.

出版信息

J Clin Microbiol. 2011 Jul;49(7):2667-70. doi: 10.1128/JCM.00328-11. Epub 2011 May 4.

DOI:10.1128/JCM.00328-11
PMID:21543570
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3147818/
Abstract

Moxalactam is highly hydrolyzed by plasmid-mediated metallo-β-lactamases (MBLs), whereas it is poorly inactivated by serine-active carbapenemases. This study demonstrated that moxalactam resistance constituted an effective screen for MBL expression in enterobacteria, which could be confirmed, even in low-MBL-producing isolates, by a disk potentiation test using moxalactam and EDTA.

摘要

美罗培南高度水解由质粒介导的金属β-内酰胺酶（MBLs），而它是由丝氨酸活性碳青霉烯酶不易失活。本研究表明，美罗培南耐药构成了肠杆菌中 MBL 表达的有效筛选，即使在低产 MBL 的分离株中，也可以通过使用美罗培南和 EDTA 的纸片增效试验来确认。

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本文引用的文献

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Possible importation and subsequent cross-transmission of OXA-48-producing Klebsiella pneumoniae, France, 2010.可能从法国输入并随后发生 OXA-48 型产酶肺炎克雷伯菌的跨种属传播，2010 年。
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NDM-1-producing Klebsiella pneumoniae isolated in the Sultanate of Oman.在阿曼苏丹国分离出产 NDM-1 的肺炎克雷伯菌。
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