使用DK/或近交系鸡进行的细胞转移研究。

Cell transfer studies with the DK/or inbred chicken lines.

作者信息

Seto F

出版信息

Adv Exp Med Biol. 1977;88:275-85. doi: 10.1007/978-1-4613-4169-7_26.

Abstract

The histoincompatibility in the DK-OR-1 (B13B13) and DK/OR-3 (B19B19) inbred White Leghorn chicken lines was assessed with the Simonsen's spleen assay, GVHR mortality counts and antibody production by transferred cells in embryonic in vivo hosts. The two lines appear to be sufficiently histocompatible for use in cell transfer experiments. The occurrence of antigen responsive cells (ARC) and precursor cells in bone marrow as assessed with the in vivo culture and baby chick immune enhancement assays. Bone marrow of unimmunized donors possessed very little anti-MRBC immune responsiveness. Bone marrow cells from MRBC-primed donors, however, showed low anti-MRBC activity in the in vivo culture which could be elevated with a second antigen stimulation. Chicks grafted as embryos with bone marrow from immunized donors responded to MRBC immunization in an enhanced manner as compared to the control untreated chicks which responded poorly if at all to the antigen. Immunization appears (a) to increase the pool of potential ARC in the bone marrow of the donor and (b) to augment the responsiveness of bone marrow cells transferred into embryonic hosts.

摘要

通过西蒙森脾脏试验、移植物抗宿主反应（GVHR）死亡率计数以及胚胎体内宿主中转输细胞的抗体产生情况，评估了DK-OR-1（B13B13）和DK/OR-3（B19B19）近交白来航鸡品系中的组织相容性。这两个品系似乎具有足够的组织相容性，可用于细胞转输实验。通过体内培养和雏鸡免疫增强试验评估了骨髓中抗原反应性细胞（ARC）和前体细胞的存在情况。未免疫供体的骨髓几乎没有抗绵羊红细胞（MRBC）免疫反应性。然而，来自经MRBC免疫的供体的骨髓细胞在体内培养中显示出低抗MRBC活性，这种活性可通过第二次抗原刺激而提高。与对照未处理的雏鸡相比，胚胎期移植了免疫供体骨髓的雏鸡对MRBC免疫的反应增强，对照雏鸡对该抗原的反应很差或根本没有反应。免疫似乎（a）增加了供体骨髓中潜在ARC的数量，并且（b）增强了转入胚胎宿主的骨髓细胞的反应性。