Laboratoire de Biologie Cellulaire et Moléculaire, Faculté des Sciences Biologiques, Université des Sciences et de la Technologie Houari Boumédienne, BP 32, El-Alia Bab Ezzouar, 16111, Algiers, Algeria.
Inflammation. 2012 Apr;35(2):501-8. doi: 10.1007/s10753-011-9338-0.
Release and activation of pro-inflammatory mediators are among the most important induced factors that are involved in the scorpion envenomation pathogenesis. Inflammatory response and lung reactivity were studied in mice following subcutaneous injection with Androctonus australis hector (Aah) venom. Venom immunodetection in lungs and sequestered cell population in the airways were determined. Cytokines, cellular peroxidase activities (eosinophil peroxidase, myeloperoxydase), and IgE antibodies were also assessed. Immunohistochemical study revealed a positive detection of the Aah venom in the alveolar wall, venule lumens, and inside inflammatory cells. Severe lung edema associated with rapid inflammatory response was observed after animal envenomation. Lung neutrophilia and eosinophilia were accompanied with IL-4, IL-5 release, and IgE synthesis. In conclusion, high cytokine levels, recruitment of inflammatory cells (eosinophils and neutrophils), and increased IgE concentration may contribute to the exacerbation and maintenance of the induced inflammatory response in lungs by scorpion venom. These results lead to the better understanding of this induced pathogenesis and could help the physicians to take care of envenomed patients.
促炎介质的释放和激活是参与蝎毒发病机制的最重要的诱导因素之一。本研究通过皮下注射安德逊非洲钳蝎（Aah）毒液，研究了小鼠的炎症反应和肺反应性。检测了肺中的毒液免疫检测和气道中隔离的细胞群。还评估了细胞因子、细胞过氧化物酶活性（嗜酸性粒细胞过氧化物酶、髓过氧化物酶）和 IgE 抗体。免疫组织化学研究显示 Aah 毒液在肺泡壁、小静脉腔和炎症细胞内呈阳性检测。动物中毒后观察到严重的肺水肿和快速的炎症反应。肺嗜中性粒细胞和嗜酸性粒细胞增多伴随着 IL-4、IL-5 的释放和 IgE 的合成。总之，高细胞因子水平、炎症细胞（嗜酸性粒细胞和中性粒细胞）的募集以及 IgE 浓度的增加可能导致蝎毒引起的肺部炎症反应的加剧和维持。这些结果有助于更好地理解这种诱导发病机制，并为医生治疗中毒患者提供帮助。
