Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, PR China.
Int J Syst Evol Microbiol. 2012 Mar;62(Pt 3):705-709. doi: 10.1099/ijs.0.031021-0. Epub 2011 May 6.
A novel alphaproteobacterial strain, designated CPCC 100156(T), was isolated from a forest soil sample collected from Hainan Island, South China, and subjected to taxonomic investigation using a polyphasic approach. The pink- to rosy-coloured colonies on TSA and YM agar were smooth and moist. Good growth occurred at 28-32 °C and at pH 7.0-7.5. The respiratory quinone was ubiquinone-9. The polar lipids consisted of phosphatidylcholine (PC), hydroxyphosphatidylethanolamine (OH-PE), phosphatidylglycerol (PG), diphosphatidylglycerol (DPG) and two unidentified aminolipids (AL1, AL2), with a minor amount of ninhydrin-positive phosphoglycolipid. (NPG). The major cellular fatty acids were summed feature 8 (C(18:1)ω7c /C(18:1)ω6c) (49.5%), summed feature 3 (C(16:1)ω7c/C(16:1)ω6c) (22.5%), and C(16:0) (14.0%). The G+C content of the genomic DNA was 70.3 mol%. The organism showed 16S rRNA gene sequence similarity of 97.37% with Belnapia moabensis DSM 16746(T). Phylogenetic analyses based on 16S rRNA gene sequences showed that the isolate belonged to the family Acetobacteraceae and consistently formed a robust cluster with Belnapia moabensis DSM 16746(T) in the phylogenetic tree. The DNA-DNA hybridization value between the new isolate and Belnapia moabensis DSM 16746(T) was 45.6%. On the basis of the taxonomic evidence, it is proposed that strain CPCC 100156(T) represents a novel species, for which the name Belnapia rosea sp. nov. is proposed. The type strain is CPCC 100156(T) (=DSM 23312(T)=CGMCC 1.10758(T)). The description of the genus Belnapia is emended accordingly.
一株新的α变形菌菌株，命名为 CPCC 100156(T)，从中国南方海南岛的森林土壤样本中分离得到，并通过多相分类方法进行了分类学研究。在 TSA 和 YM 琼脂上，粉红色到玫瑰色的菌落光滑湿润。在 28-32°C 和 pH 值 7.0-7.5 的条件下生长良好。呼吸醌为泛醌-9。极性脂类由磷脂酰胆碱 (PC)、羟基磷酯乙醇胺 (OH-PE)、磷脂酰甘油 (PG)、双磷脂酰甘油 (DPG) 和两种未鉴定的胺脂 (AL1、AL2) 组成，还有少量的水合胲阳性磷酯糖脂 (NPG)。主要的细胞脂肪酸是总和特征 8 (C(18:1)ω7c/C(18:1)ω6c) (49.5%)、总和特征 3 (C(16:1)ω7c/C(16:1)ω6c) (22.5%)和 C(16:0) (14.0%)。基因组 DNA 的 G+C 含量为 70.3 mol%。该菌株与 Belnapia moabensis DSM 16746(T) 的 16S rRNA 基因序列相似度为 97.37%。基于 16S rRNA 基因序列的系统发育分析表明，该分离株属于醋酸杆菌科，在系统发育树中与 Belnapia moabensis DSM 16746(T) 形成一个稳健的聚类。新分离株与 Belnapia moabensis DSM 16746(T) 的 DNA-DNA 杂交值为 45.6%。基于分类学证据，建议将菌株 CPCC 100156(T) 代表一个新的种，命名为 Belnapia rosea sp. nov.。模式菌株为 CPCC 100156(T)=DSM 23312(T)=CGMCC 1.10758(T)。相应地对 Belnapia 属的描述进行了修正。
