Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung Marine University, no. 142, Hai-Chuan Rd, Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
Department of Marine Biotechnology, National Kaohsiung Marine University, no. 142, Hai-Chuan Rd, Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
Int J Syst Evol Microbiol. 2012 Mar;62(Pt 3):698-704. doi: 10.1099/ijs.0.030635-0. Epub 2011 May 6.
A Gram-negative, facultatively anaerobic, short-rod-shaped, non-motile and non-spore-forming bacterial strain, designated ABP-4(T), was isolated from a freshwater spring in Taiwan and was characterized using the polyphasic taxonomy approach. Growth occurred at 20-40 °C (optimum, 30-37 °C), at pH 7.0-10.0 (optimum, pH 7.0-9.0) and with 0-3% NaCl (optimum, 0%). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain ABP-4(T), together with Aquabacterium fontiphilum CS-6(T) (96.4% sequence similarity), Aquabacterium commune B8(T) (96.1%), Aquabacterium citratiphilum B4(T) (95.5%) and Aquabacterium parvum B6(T) (94.7%), formed a deep line within the order Burkholderiales. Strain ABP-4(T) contained summed feature 3 (comprising C(16:1) ω7c and/or C(16:1) ω6c), C(18:1) ω7c and C(16:0) as predominant fatty acids. The major cellular hydroxy fatty acid was C(10:0) 3-OH. The major isoprenoid quinone was Q-8 and the DNA G+C content was 68.6 mol%. The polar lipid profile consisted of a mixture of phosphatidylethanolamine, phosphatidylglycerol, phosphatidylserine, diphosphatidylglycerol and several uncharacterized phospholipids. The DNA-DNA relatedness of strain ABP-4(T) with respect to recognized species of the genus Aquabacterium was less than 70%. On the basis of the genotypic, chemotaxonomic and phenotypic data, strain ABP-4(T) represents a novel species in the genus Aquabacterium, for which the name Aquabacterium limnoticum sp. nov. is proposed. The type strain is ABP-4(T) (=BCRC 80167(T)=KCTC 23306(T)).
一株革兰氏阴性、兼性厌氧、短杆状、无动力、非孢子形成的细菌菌株，被命名为 ABP-4(T)，从台湾的一个淡水泉中分离出来，并采用多相分类学方法进行了表征。该菌株在 20-40°C（最适温度为 30-37°C）、pH7.0-10.0（最适 pH7.0-9.0）和 0-3%NaCl（最适 0%）的条件下生长。基于 16S rRNA 基因序列的系统发育分析表明，菌株 ABP-4(T)与 Aquabacterium fontiphilum CS-6(T)（96.4%序列相似性）、Aquabacterium commune B8(T)（96.1%）、Aquabacterium citratiphilum B4(T)（95.5%）和 Aquabacterium parvum B6(T)（94.7%）一起，在伯克霍尔德氏菌目内形成了一个深分支。菌株 ABP-4(T)含有特征性脂肪酸 3（包含 C(16:1) ω7c 和/或 C(16:1) ω6c）、C(18:1) ω7c 和 C(16:0)，为主脂肪酸。主要的细胞羟基脂肪酸为 C(10:0) 3-OH。主要的异戊烯醌为 Q-8，DNA G+C 含量为 68.6mol%。极性脂谱由磷脂酰乙醇胺、磷脂酰甘油、磷脂酰丝氨酸、双磷脂酰甘油和几种未鉴定的磷脂组成。菌株 ABP-4(T)与 Aquabacterium 属的已知种的 DNA-DNA 同源性小于 70%。基于基因型、化学分类和表型数据，菌株 ABP-4(T)代表 Aquabacterium 属的一个新种，建议将其命名为 Aquabacterium limnoticum sp. nov.。模式菌株为 ABP-4(T)=BCRC 80167=KCTC 23306。
