Department of Parasitology, Kangwon National University School of Medicine, Hyoja2-dong, Chuncheon, Gangwon-do 200-701, Republic of Korea.
Parasitol Res. 2011 Dec;109(6):1571-6. doi: 10.1007/s00436-011-2413-5. Epub 2011 May 10.
The Plasmodium vivax merozoite surface protein 1 (Pvmsp-1) locus codes for a major asexual blood-stage antigen currently proposed as a malaria vaccine candidate antigen. However, extensive polymorphism of this protein has been observed in isolates from different geographical areas. Here, we investigate the extent and the frequency of allelic diversity at the Pvmsp-1 locus in field isolates collected in the Republic of Korea during the past decade. Among the 45 Korean isolates, six Pvmsp-1 gene types (SKOR-I to SKOR-VI) were identified as unique combinations of type sequences in each variable block. Of these six different Pvmsp-1 gene types, two major Pvmsp-1 allelic types were found in 72% (SKOR-I) and 28% (SKOR-II) of field isolates collected in 1996 to 2000, and four different allelic types (SKOR-III to SKOR-VI) emerged in 70% (10-25%) of isolates collected in 2007 to 2009. These results suggest that allelic diversity of Pvmsp-1 increased in several variable regions, including the N- and C-terminals, after reemergence of P. vivax parasites in the Republic of Korea.
恶性疟原虫裂殖子表面蛋白 1（Pvmsp-1）基因座编码一种主要的无性血期抗原，目前被提议作为疟疾疫苗候选抗原。然而，已经在来自不同地理区域的分离株中观察到这种蛋白的广泛多态性。在这里，我们研究了过去十年在韩国共和国采集的现场分离株中 Pvmsp-1 基因座的等位基因多样性的程度和频率。在 45 个韩国分离株中，确定了 6 种 Pvmsp-1 基因类型（SKOR-I 至 SKOR-VI），它们是每个可变块中类型序列的独特组合。在 1996 年至 2000 年采集的 72%（SKOR-I）和 28%（SKOR-II）的现场分离株中发现了两种主要的 Pvmsp-1 等位基因类型，而在 2007 年至 2009 年采集的 70%（10-25%）的分离株中出现了四种不同的等位基因类型（SKOR-III 至 SKOR-VI）。这些结果表明，在韩国重新出现恶性疟原虫寄生虫后，Pvmsp-1 的等位基因多样性在包括 N 和 C 末端在内的几个可变区增加。
