Max Planck Institute for Developmental Biology, 72076, Tübingen, Germany.
Adv Exp Med Biol. 2011;715:257-69. doi: 10.1007/978-94-007-0940-9_16.
Since its introduction 70 years ago electron microscopy has become an invaluable tool for microbiology and the study of bacterial interaction. Technological development over the past decades has enabled researchers to resolve smaller and smaller details in bacterial samples, while new preparation techniques like cryo preparation now allow to investigate bacteria even closer to their natural state. In this chapter we give a brief overview of electron microscopy techniques suitable for the investigation of bacterial adhesion at molecular as well as cellular level and a short outlook on future technologies relevant to the field.
自 70 年前问世以来，电子显微镜已成为微生物学和细菌相互作用研究的宝贵工具。过去几十年来的技术发展使研究人员能够解析细菌样本中越来越小的细节，而像 cryo preparation 这样的新制备技术现在甚至可以让他们更接近细菌的自然状态来进行研究。在本章中，我们简要概述了适用于在分子和细胞水平上研究细菌黏附的电子显微镜技术，并对该领域相关的未来技术进行了简短展望。
