State Key Laboratory of Emerging Infectious Diseases, Hong Kong; Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong.
Diagn Microbiol Infect Dis. 2011 Jul;70(3):316-23. doi: 10.1016/j.diagmicrobio.2011.03.006. Epub 2011 May 10.
Although multilocus sequence typing (MLST) has been widely used for bacterial typing, the contribution of the gene loci to the discriminatory power of each MLST scheme is unknown. We analyzed the discriminatory powers of 36 MLST schemes using all combinations of the 7 loci and contributions of each locus to the schemes. In 10 schemes, sequencing 6 loci can achieve the discriminatory powers of 7 loci. For the other 26 schemes, the median marginal increase in discriminatory power when 7 instead of 6 loci were used is 0.0004. Sequencing the 7 loci of 50 strains each of Pseudomonas aeruginosa and Acinetobacter baumannii revealed that the discriminatory power for P. aeruginosa was 0.9861 when either 6 (without trp) or 7 loci were used and that for A. baumannii was 0.9363 when 5, 6, or 7 loci were used. Genes that have no additional or minimal contribution to the overall discriminatory powers should be replaced.
虽然多位点序列分型（MLST）已被广泛用于细菌分型，但各 MLST 方案中基因座对其分辨力的贡献尚不清楚。我们分析了 36 种 MLST 方案的分辨力，使用了 7 个基因座的所有组合以及每个基因座对方案的贡献。在 10 个方案中，测序 6 个基因座即可达到 7 个基因座的分辨力。对于其他 26 个方案，当使用 7 个而不是 6 个基因座时，分辨力的中位数边际增加量为 0.0004。对 50 株铜绿假单胞菌和鲍曼不动杆菌的每株菌株的 7 个基因座进行测序，结果显示，当使用 6 个（不含 trp）或 7 个基因座时，铜绿假单胞菌的分辨力为 0.9861，当使用 5、6 或 7 个基因座时，鲍曼不动杆菌的分辨力为 0.9363。对整体分辨力没有额外或最小贡献的基因座应被替换。
