开发一种扩展的多位点序列分型方法，用于对布鲁氏菌分离株进行基因分型。
Development of an extended multilocus sequence typing for genotyping of Brucella isolates.
机构信息
School of Public Health, Key Laboratory of Zoonosis, Ministry of Education, Jilin University, Changchun, Jilin 130021, PR China.
出版信息
J Microbiol Methods. 2011 Aug;86(2):252-4. doi: 10.1016/j.mimet.2011.05.013. Epub 2011 May 27.
PMID:21640765
Abstract
By amplifying and sequencing longer sequences, an extended multi locus sequence typing (EMLST) theme was developed for Brucella. 61 isolates were genotyped by the EMLST with increased resolution. This strategy could be extended to other bacteria to improve MLST genotyping resolution without additional loci.
摘要
通过扩增和测序更长的序列，为布鲁氏菌开发了扩展多位点序列分型（EMLST）主题。61 株分离株通过 EMLST 进行了基因分型，分辨率提高。该策略可扩展到其他细菌，在不增加基因座的情况下提高 MLST 基因分型分辨率。
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