Wellcome Trust Centre for Cell-Matrix Research, Faculty of Life Sciences, University of Manchester, Manchester M13 9PT, United Kingdom.
J Biol Chem. 2011 Jul 15;286(28):24987-95. doi: 10.1074/jbc.M111.242263. Epub 2011 May 11.
The microtubule (MT) and actin cytoskeletons are fundamental to cell integrity, because they control a host of cellular activities, including cell division, growth, polarization, and migration. Proteins involved in mediating the cross-talk between MT and actin cytoskeletons are key to many cellular processes and play important physiological roles. We identified a new member of the GAS2 family of MT-actin cross-linking proteins, named G2L3 (GAS2-like 3). We show that GAS2-like 3 is widely conserved throughout evolution and is ubiquitously expressed in human tissues. GAS2-like 3 interacts with filamentous actin and MTs via its single calponin homology type 3 domain and C terminus, respectively. Interestingly, the role of the putative MT-binding GAS2-related domain is to modulate the binding of GAS2-like 3 to both filamentous actin and MTs. This is in contrast to GAS2-related domains found in related proteins, where it functions as a MT-binding domain. Furthermore, we show that tubulin acetylation drives GAS2-like 3 localization to MTs and may provide functional insights into the role of GAS2-like 3.
微管（MT）和肌动蛋白细胞骨架对于细胞完整性至关重要，因为它们控制着许多细胞活动，包括细胞分裂、生长、极化和迁移。参与调节 MT 和肌动蛋白细胞骨架之间串扰的蛋白质是许多细胞过程的关键，发挥着重要的生理作用。我们鉴定了一种新的微管-肌动蛋白交联蛋白 GAS2 家族成员，命名为 G2L3（GAS2 样 3）。我们表明，GAS2 样 3 在进化过程中广泛保守，并在人类组织中普遍表达。GAS2 样 3 通过其单个钙调蛋白同源结构域 3 和 C 末端分别与丝状肌动蛋白和 MT 相互作用。有趣的是，假定的 MT 结合 GAS2 相关结构域的作用是调节 GAS2 样 3 与丝状肌动蛋白和 MT 的结合。这与在相关蛋白中发现的 GAS2 相关结构域形成对比，后者作为 MT 结合结构域发挥作用。此外，我们表明微管蛋白乙酰化驱动 GAS2 样 3 向 MT 的定位，这可能为 GAS2 样 3 的作用提供功能见解。
