宿主细胞对两种牛病毒性腹泻病毒毒株体外特性表达的影响。

Effect of host cell on the in vitro characteristics expressed by two bovine viral diarrhea virus strains.

作者信息

Johnson J C, Rosenbusch R F

机构信息

Schering-Plough Animal Health, Omaha, NE 68103.

出版信息

Vet Microbiol. 1990 Feb;21(4):319-28. doi: 10.1016/0378-1135(90)90003-e.

DOI:10.1016/0378-1135(90)90003-e

PMID:2156373

Abstract

Growth curves, immunofluorescence assays and radioimmunoprecipitation analysis of bovine viral diarrhea virus (BVDV)-specified polypeptides were used to determine the susceptibility of bovine endothelial cells (BECs) to infection with strains of BVDV. One strain of the virus, NY1, normally non-cytopathic when grown in bovine turbinate cells (BTUs), did not parallel this type behavior in BECs. Virus replication kinetics, morphological changes in infected cells and resolution of viral polypeptides suggest that NY1 replicated and showed cytopathologic changes in infected BECs similar to a cytopathic strain of BVDV, strain C24V. Two previously unreported viral polypeptides were identified in NY1-infected endothelial cells. These polypeptides had molecular weights of 92,000 (92kDa) and 72kDa. These findings suggest that BVDV-cell interactions occurring in BECs differ from those observed in similarly infected BTUs.

摘要

利用生长曲线、免疫荧光测定以及牛病毒性腹泻病毒（BVDV）特异性多肽的放射免疫沉淀分析，来确定牛内皮细胞（BECs）对BVDV毒株感染的易感性。该病毒的一个毒株NY1，在牛鼻甲细胞（BTUs）中生长时通常无细胞病变，但在BECs中却不呈现这种行为模式。病毒复制动力学、受感染细胞的形态变化以及病毒多肽的解析表明，NY1在受感染的BECs中进行复制，并呈现出细胞病变变化，类似于BVDV的细胞病变毒株C24V。在NY1感染的内皮细胞中鉴定出两种先前未报道的病毒多肽。这些多肽的分子量分别为92,000（92kDa）和72kDa。这些发现表明，BECs中发生的BVDV与细胞的相互作用不同于在类似感染的BTUs中观察到的相互作用。