Key Laboratory of MOE for Plant Development Biology, College of Life Sciences, Wuhan University, Wuhan 430072, China Wuhan National Field Observation & Research Station for Aquatic Vegetables, Wuhan 430065, China.
Mol Ecol Resour. 2009 May;9(3):887-9. doi: 10.1111/j.1755-0998.2008.02357.x. Epub 2009 Feb 3.
Sixteen polymorphic microsatellite markers were isolated and identified in Zizania latifolia Turcz. (Poaceae), a perennial aquatic plant widespread in Eastern Asia. The microsatellite-enriched library was constructed using the fast isolation by AFLP of sequences containing repeats method. These markers revealed two to 14 alleles, with an average of 5.6 alleles per locus. The observed and expected heterozygosities varied from 0.071 to 0.690 and from 0.174 to 0.812, respectively. These markers will be useful for studying of gene flow and evaluating the genetic diversity of the Zizania latifolia population.
十六个多态性微卫星标记被分离并鉴定于菰(禾本科),一种广泛分布于东亚的多年生水生植物。微卫星富集文库使用 AFLP 序列中重复的快速分离方法构建。这些标记显示每个位点有 2 到 14 个等位基因,平均每个位点有 5.6 个等位基因。观测到的和预期的杂合度分别从 0.071 到 0.690 和从 0.174 到 0.812 不等。这些标记将有助于研究基因流和评估菰种群的遗传多样性。