Ocean College, Hainan University, Key Laboratory of Tropic Biological Resources, MOE, Hainan Key Laboratory of Tropical Hydrobiological Technology, 58 Renmin Road, Haikou, Hainan 570228, China.
Mol Ecol Resour. 2009 Jul;9(4):1264-7. doi: 10.1111/j.1755-0998.2009.02660.x. Epub 2009 Apr 7.
Thirty polymorphic microsatellite markers were developed for the Atlantic surfclam, Spisula solidissima, from an enriched library and characterized in 24 clams from a wild population. The number of alleles ranged from 3 to 16 per locus. The expected and observed heterozygosities ranged from 0.1942 to 0.9238 and 0.0833 to 0.875 respectively. Six loci showed significant (P < 0.05 after Bonferroni correction) deviation from Hardy-Weinberg equilibrium, probably because of the presence of null alleles. Three primer pairs amplified duplicated loci with two involving tandem mini-satellite repeats. Most of the microsatellite markers developed here should be useful for genetic studies in this species.
从富集文库中为大西洋美洲帘蛤（Spisula solidissima）开发了 30 个多态微卫星标记，并在野生种群的 24 个蛤中进行了特征分析。每个位点的等位基因数范围为 3 到 16。预期和观察到的杂合度分别为 0.1942 到 0.9238 和 0.0833 到 0.875。6 个位点表现出与 Hardy-Weinberg 平衡显著偏离（经 Bonferroni 校正后 P<0.05），可能是因为存在无效等位基因。有 3 对引物对扩增了重复的位点，其中有 2 对涉及串联的微卫星重复。这里开发的大多数微卫星标记应该对该物种的遗传研究有用。
