IGBMC (Institut de Génétique et de Biologie Moléculaire et Cellulaire), CNRS, UMR7104, Inserm, U596, Collège de France, Strasbourg University, Illkirch-Graffenstaden 67404, France.
EMBO Rep. 2011 Jul 1;12(7):697-704. doi: 10.1038/embor.2011.76.
Targeting of messenger RNAs (mRNAs) in neuron processes relies on cis-acting regulatory elements, the nature of which is poorly understood. Here, we report that approximately 30% of the best-known dendritic mRNAs contain a guanine (G)-quadruplex consensus in their 3'-untranslated region. Among these mRNAs, we show by using RNA structure probing that a G-quadruplex is present in the mRNAs of two key postsynaptic proteins: PSD-95 and CaMKIIa. The G-quadruplex structure is necessary and sufficient for the potent and fast localization of mRNAs in cortical neurites and this occurs in a metabotropic glutamate receptor-responsive manner. Thus, G-quadruplex seems to be a common neurite localization signal.
靶向神经元过程中的信使 RNA（mRNA）依赖于顺式作用调节元件，但这些元件的性质尚未完全了解。在这里，我们报告说，大约 30%的最著名的树突状 mRNA 在其 3'非翻译区含有鸟嘌呤（G）四链体的共识序列。在这些 mRNA 中，我们通过使用 RNA 结构探测显示，两个关键的突触后蛋白 PSD-95 和 CaMKIIa 的 mRNA 中存在 G-四链体。G-四链体结构对于 mRNA 在皮质神经元突中的有效和快速定位是必需和充分的，这种定位方式发生在代谢型谷氨酸受体反应的方式。因此，G-四链体似乎是一种常见的神经元突定位信号。
