Centro de Pesquisa em Virologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Av Bandeirantes 3900, Monte Alegre, Ribeirão Preto, SP 14090-900, Brazil.
Virol J. 2011 May 11;8:218. doi: 10.1186/1743-422X-8-218.
Antigens for Hantavirus serological tests have been produced using DNA recombinant technology for more than twenty years. Several different strategies have been used for that purpose. All of them avoid the risks and difficulties involved in multiplying Hantavirus in the laboratory. In Brazil, the Araraquara virus is one of the main causes of Hantavirus Cardio-Pulmonary Syndrome (HCPS).
In this investigation, we report the expression of the N protein of the Araraquara Hantavirus in a Baculovirus Expression System, the use of this protein in IgM and IgG ELISA and comparison with the same antigen generated in E. coli.
The protein obtained, and purified in a nickel column, was effectively recognized by antibodies from confirmed HCPS patients. Comparison of the baculovirus generated antigen with the N protein produced in E. coli showed that both were equally effective in terms of sensitivity and specificity.
Our results therefore indicate that either of these proteins can be used in serological tests in Brazil.
抗汉坦病毒血清学检测用的抗原已经使用 DNA 重组技术生产了二十多年。为此目的已经使用了几种不同的策略。所有这些策略都避免了在实验室中繁殖汉坦病毒所涉及的风险和困难。在巴西，阿雷亚夸拉病毒是汉坦病毒心肺综合征（HCPS）的主要原因之一。
在这项研究中，我们报告了在杆状病毒表达系统中表达阿雷亚夸拉汉坦病毒的 N 蛋白，以及该蛋白在 IgM 和 IgG ELISA 中的应用，并与在大肠杆菌中生成的相同抗原进行了比较。
用镍柱纯化的蛋白能被确诊为 HCPS 患者的抗体有效识别。杆状病毒生成的抗原与在大肠杆菌中产生的 N 蛋白的比较表明，这两种抗原在敏感性和特异性方面同样有效。
因此，我们的结果表明，这两种蛋白中的任何一种都可以在巴西的血清学检测中使用。
