基于阿拉拉夸拉病毒重组核衣壳蛋白的酶联免疫吸附测定法的评估
Evaluation of an enzyme-linked immunosorbent assay based on Araraquara virus recombinant nucleocapsid protein.
作者信息
Figueiredo Luiz Tadeu Moraes, Moreli Marcos Lazaro, Borges Alessandra Abel, de Figueiredo Glauciane Garcia, Badra Soraya Jabur, Bisordi Ivani, Suzuki Akemi, Capria Silvana, Padula Paula
机构信息
Virology Research Center, School of Medicine of the University of São Paulo in Ribeirão Preto, Ribeirão Preto, Brazil.
出版信息
Am J Trop Med Hyg. 2009 Aug;81(2):273-6.
Laboratory diagnosis of hantavirus cardiopulmonary syndrome (HCPS) in Brazil has been performed mostly by detection of IgM antibodies to recombinant antigen purified from Sin Nombre virus and Andes virus (ANDV). Recently, a recombinant nucleocapsid (rN) protein of Araraquara virus (ARAV), a Brazilian hantavirus, was obtained in Escherichia coli. To evaluate ARAV rN as antigen for antibody detection, serum samples from 30 patients from Argentina seropositive for hantavirus were tested. All samples were positive for IgG and IgM by enzyme-linked immunosorbent assay (ELISA) using either ARAV rN or ANDV rN antigens. In Brazil, six of 60 serum samples from patients with suspected HCPS (10%) were positive for IgM by ELISA using ARAV rN antigen and 7 were positive using ANDV rN antigen. For results obtained with 90 serum samples analyzed by IgM ELISA with ANDV rN antigen, the sensitivity of the IgM ELISA using ARAV rN antigen was 97.2%, the specificity was 100%, the positive predictive value was 100%, and the negative predictive value was 98.1%. The results show that ARAV rN is a suitable antigen for diagnosis of hantavirus infection in Brazil and Argentina.
巴西汉坦病毒心肺综合征（HCPS）的实验室诊断大多是通过检测针对从辛诺柏病毒和安第斯病毒（ANDV）纯化的重组抗原的IgM抗体来进行的。最近，在大肠杆菌中获得了一种巴西汉坦病毒——阿拉拉夸拉病毒（ARAV）的重组核衣壳（rN）蛋白。为了评估ARAV rN作为抗体检测抗原的效果，对来自阿根廷的30例汉坦病毒血清学阳性患者的血清样本进行了检测。使用ARAV rN或ANDV rN抗原通过酶联免疫吸附测定（ELISA）检测，所有样本的IgG和IgM均呈阳性。在巴西，60例疑似HCPS患者的血清样本中，有6例（10%）使用ARAV rN抗原通过ELISA检测IgM呈阳性，7例使用ANDV rN抗原呈阳性。对于使用ANDV rN抗原通过IgM ELISA分析的90份血清样本所获得的结果，使用ARAV rN抗原的IgM ELISA的敏感性为97.2%，特异性为100%，阳性预测值为100%，阴性预测值为98.1%。结果表明，ARAV rN是巴西和阿根廷诊断汉坦病毒感染的合适抗原。
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