Department of Life and Nanopharmaceutical Sciences and Department of Biology, Kyung Hee University, Seoul 130-701, Republic of Korea.
Int J Syst Evol Microbiol. 2012 Apr;62(Pt 4):776-779. doi: 10.1099/ijs.0.031625-0. Epub 2011 May 13.
Strain GAM6-1T is a novel, strictly anaerobic, non-spore-forming, Gram-stain-positive bacterium that was isolated from the faeces of a healthy individual. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain GAM6-1T was most closely related to Blautia producta ATCC 27340T (95.7 % sequence similarity), in the family Lachnospiraceae. Strain GAM6-1T did not exhibit catalase or oxidase activity. The strain's cellular fatty acids were of the straight-chain saturated and mono-unsaturated types, with C14:0 (24.10 %), C16:0 (19.09 %) and C16:0 dimethylacetal (14.35 %) predominant. Strain GAM6-1T was able to produce acid from various carbohydrates. Glucose fermentation produced acetic acid as the major short-chain fatty acid. The genomic DNA G+C content of strain GAM6-1T was 35.6 mol%. Based on phenotypic, genotypic and phylogenetic evidence, strain GAM6-1T (=KCTC 5981T=JCM 17204T) is considered to represent a novel species, for which the name Blautia stercoris sp. nov. is proposed.
菌株 GAM6-1T 是一株新型的、严格厌氧、非孢子形成、革兰氏阳性细菌，从一个健康个体的粪便中分离得到。基于 16S rRNA 基因序列的系统发育分析表明，菌株 GAM6-1T 与布劳特氏菌属的 Blautia producta ATCC 27340T（相似度为 95.7%）最为密切相关，同属于毛螺菌科。菌株 GAM6-1T 不表现出过氧化氢酶或氧化酶活性。该菌株的细胞脂肪酸属于直链饱和和单不饱和类型，主要有 C14:0（24.10%）、C16:0（19.09%）和 C16:0 二甲缩醛（14.35%）。菌株 GAM6-1T 能够从各种碳水化合物中产生酸。葡萄糖发酵产生的主要短链脂肪酸是乙酸。菌株 GAM6-1T 的基因组 DNA G+C 含量为 35.6mol%。基于表型、基因型和系统发育证据，菌株 GAM6-1T（=KCTC 5981T=JCM 17204T）被认为代表了一个新的物种，建议将其命名为 Stercoris 布劳特氏菌（Blautia stercoris）sp. nov.。
