Kläring Karoline, Just Sarah, Lagkouvardos Ilias, Hanske Laura, Haller Dirk, Blaut Michael, Wenning Mareike, Clavel Thomas
Department of Gastrointestinal Microbiology, German Institute of Human Nutrition Potsdam-Rehbrücke, Nuthetal, Germany.
Junior Research Group Intestinal Microbiome, ZIEL - Research Center for Nutrition and Food Sciences, Technische Universität München, Freising-Weihenstephan, Germany.
Int J Syst Evol Microbiol. 2015 Mar;65(Pt 3):870-878. doi: 10.1099/ijs.0.000030. Epub 2014 Dec 17.
Three strains of an anaerobic, Gram-stain-positive coccobacillus were isolated from the intestines of mice. These strains shared 100 % similarity in their 16S rRNA gene sequences, but were distantly related to any described members of the family Lachnospiraceae (<94 %). The most closely related species with names that have standing in nomenclature were Robinsoniella peoriensis, Ruminococcus gnavus, Blautia producta and Clostridium xylanolyticum. Phylogenetic relationships based on 16S rRNA gene sequence analysis were confirmed by partial sequencing of hsp60 genes. The use of an in-house database search pipeline revealed that the new isolates are most prevalent in bovine gut samples when compared with human and mouse samples for Ruminococcus gnavus and B. producta. All three isolated strains shared similar cellular fatty acid patterns dominated by C16 : 0 methyl ester. Differences in the proportions of C12 : 0 methyl ester, C14 : 0 methyl ester and C18 : 1 cis-11 dimethyl acetal were observed when compared with phylogenetically neighbouring species. The major short-chain fatty acid produced by strain SRB-530-5-H(T) was acetic acid. This strain tested positive for utilization of d-fructose, d-galacturonic acid, d-malic acid, l-alanyl l-threonine and l-glutamic acid but was negative for utilization of amygdalin, arbutin, α-d-glucose, 3-methyl d-glucose and salicin, in contrast to the type strain of the closest related species Robinsoniella peoriensis. The isolates were not able to use mannitol for growth. Based on genotypic, phenotypic and chemotaxonomic characteristics, we propose to create the new genus and species Murimonas intestini gen. nov., sp. nov. to accommodate the three strains SRB-530-5-H(T) ( = DSM 26524(T) = CCUG 63391(T)) (the type strain of Murimonas intestini), SRB-509-4-S-H ( = DSM 27577 = CCUG 64595) and SRB-524-4-S-H ( = DSM 27578 = CCUG 64594).
从小鼠肠道中分离出三株厌氧、革兰氏染色阳性的球杆菌。这些菌株的16S rRNA基因序列相似度为100%，但与毛螺菌科的任何已描述成员关系较远（<94%）。在具有命名地位的物种中，与其亲缘关系最近的是皮奥里亚罗宾逊菌、解糖瘤胃球菌、产丁酸布劳特氏菌和木聚糖分解梭菌。基于hsp60基因的部分测序，证实了基于16S rRNA基因序列分析的系统发育关系。使用内部数据库搜索流程发现，与人类和小鼠样本相比，在牛肠道样本中，新分离株在解糖瘤胃球菌和产丁酸布劳特氏菌方面最为常见。所有三株分离菌株都具有相似的细胞脂肪酸模式，以C16 : 0甲酯为主。与系统发育上相邻的物种相比，观察到C12 : 0甲酯、C14 : 0甲酯和C18 : 1顺式-11二甲基缩醛比例存在差异。菌株SRB-530-5-H(T)产生的主要短链脂肪酸是乙酸。与亲缘关系最近的物种皮奥里亚罗宾逊菌的模式菌株相比，该菌株对d-果糖、d-半乳糖醛酸、d-苹果酸、l-丙氨酰-l-苏氨酸和l-谷氨酸的利用测试呈阳性，但对扁桃苷、熊果苷、α-d-葡萄糖、3-甲基-d-葡萄糖和水杨苷的利用测试呈阴性。这些分离株不能利用甘露醇生长。基于基因型、表型和化学分类学特征，我们提议创建新属新种肠道鼠单胞菌Murimonas intestini gen. nov., sp. nov.，以容纳这三株菌株SRB-530-5-H(T)（=DSM 26524(T)=CCUG 63391(T)）（肠道鼠单胞菌的模式菌株）、SRB-509-4-S-H（=DSM 27577=CCUG 64595）和SRB-524-4-S-H（=DSM 27578=CCUG 64594）。
