建立并应用一种简单的环介导等温扩增方法快速检测李斯特菌。
Development and application of a simple loop-mediated isothermal amplification method on rapid detection of Listeria monocytogenes strains.
机构信息
Food Safety Key Laboratory of Guangdong Province, College of Food Science, South China Agricultural University, Guangzhou, 510642, China.
出版信息
Mol Biol Rep. 2012 Jan;39(1):445-9. doi: 10.1007/s11033-011-0757-7. Epub 2011 May 14.
A loop-mediated isothermal amplification (LAMP) method for rapid detection of the food-borne L. monocytogenes strains had been developed and evaluated in this study. The optimal reaction condition was 65 °C for 45 min, with the detection limit as 1 pg DNA/tube and 100 CFU/reaction. Application of the established LAMP assay was performed on 182 food-borne L. monocytogenes strains using a rapid procedure and easy result confirmation, with the sensitivity of LAMP versus PCR assays as 96.7% (176/182) and 91.2% (166/182), respectively; with 100% specificity, positive predictive value (PPV) and negative predictive value (NPV) for both assays.
本研究中开发并评估了一种用于快速检测食源性病原体单核细胞增生李斯特菌的环介导等温扩增（LAMP）方法。最佳反应条件为 65°C 45 分钟，检测限为 1pg DNA/管和 100CFU/反应。应用建立的 LAMP 检测方法对 182 株食源性病原体单核细胞增生李斯特菌进行了快速检测，结果易于确认，LAMP 与 PCR 检测方法的灵敏度分别为 96.7%（176/182）和 91.2%（166/182）；两种方法均具有 100%的特异性、阳性预测值（PPV）和阴性预测值（NPV）。
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