Kalekou Harikleia, Miliaras Dimosthenis
Department of Stomatology, School of Dentistry, Aristotle University, Thessaloniki, Greece.
Pol J Pathol. 2011;62(1):25-30.
Cytokeratin expression is being frequently used for the differential diagnosis of carcinomas originating from different sites. Among the various cytokeratins, the combination of cytokeratins (CKs) 7 and 20 is considered to be the most useful for this purpose. However, there are very few reports in the literature regarding CK7 and CK20 expression in gallbladder carcinoma (GBC). In this paper we studied the immunohistochemical expression of CK7 and CK20 in 42 GBC cases. In addition, we studied 25 randomly selected cases of lithiasis associated chronic cholecystitis (LaCC) as controls. CK7/CK20 immunoprofile was assessed in relation to tumour differentiation, depth of invasion, and p53 protein expression. Twenty-nine GBC cases (69.05%) were CK7-positive, and 12 cases (28.57%) were CK20-positive. Tumour differentiation was not correlated with CK7 or CK20 immunoreaction. Regarding tumour depth of invasion, the CK7+/CK20- group presented a significant correlation with early stage (T1) disease (p = 0.04). p53 expression was correlated with both CK7 (p = 0.05) and CK20 (p = 0.023) expression. All the cases of LaCC demonstrated diffuse intense CK7 positivity of the mucosal epithelium, while CK20 was only focally positive in 13/25 cases. Our results along with the data from the literature indicate that CK7/CK20 expression may be of clinical significance, and further investigation in this direction is needed.
细胞角蛋白表达常用于不同部位起源的癌的鉴别诊断。在各种细胞角蛋白中，细胞角蛋白（CKs）7和20的联合被认为对此目的最有用。然而，文献中关于胆囊癌（GBC）中CK7和CK20表达的报道非常少。在本文中，我们研究了42例GBC病例中CK7和CK20的免疫组化表达。此外，我们随机选取了25例结石相关性慢性胆囊炎（LaCC）病例作为对照。评估了CK7/CK20免疫表型与肿瘤分化、浸润深度和p53蛋白表达的关系。29例GBC病例（69.05%）CK7阳性，12例（28.57%）CK20阳性。肿瘤分化与CK7或CK20免疫反应无关。关于肿瘤浸润深度，CK7+/CK20-组与早期（T1）疾病显著相关（p = 0.04）。p53表达与CK7（p = 0.05）和CK20（p = 0.023）表达均相关。所有LaCC病例均显示黏膜上皮弥漫性强CK7阳性，而CK20仅在13/25例中局灶性阳性。我们的结果以及文献数据表明CK7/CK20表达可能具有临床意义，需要在这个方向上进一步研究。
