开发小鲍螺（Haliotis diversicolor Reeve）的 11 个多态性微卫星标记。

Development of 11 polymorphic microsatellite loci in the small abalone (Haliotis diversicolor Reeve).

机构信息

Key Laboratory of Science and Technology for Aquaculture and Food Safety, Fisheries College/Fisheries Biotechnology Institute, Jimei University, Xiamen 361021, China Department of Oceanography, Xiamen University, Xiamen 361005, China.

出版信息

Mol Ecol Resour. 2008 Nov;8(6):1390-2. doi: 10.1111/j.1755-0998.2008.02329.x.

DOI:10.1111/j.1755-0998.2008.02329.x

PMID:21586054

Abstract

Eleven polymorphic microsatellite loci for Haliotis diversicolor were isolated and characterized from (CT)(n) - and (AC)(n) -enriched library. They were tested in 24 individuals from a natural population. All of them were polymorphic, with the number of alleles varying between three and 10. The observed heterozygosities and expected heterozygosities ranged from 0.083 to 0.913 and from 0.159 to 0.856, respectively. The 11 isolated microsatellite loci, except SA-JMU6 and SA-JMU12, followed Hardy-Weinberg expectations. Seven sets of primers also amplify in closely related species, H. ovina and H. asinine. These 11 polymorphic microsatellites will be useful for analysing the population structure and genetic diversity in H. diversicolor.

摘要

从富含 (CT)(n) 和 (AC)(n) 的文库中分离和鉴定了 11 个多态性微卫星标记用于杂色鲍。在来自自然种群的 24 个个体中对它们进行了测试。所有这些标记均为多态性的，等位基因数量从 3 到 10 不等。观察到的杂合度和预期杂合度分别在 0.083 到 0.913 之间和 0.159 到 0.856 之间。11 个分离的微卫星标记，除了 SA-JMU6 和 SA-JMU12 外，均符合 Hardy-Weinberg 预期。七对引物也可以在近缘种，即欧洲鲍和皱纹盘鲍中扩增。这 11 个多态性微卫星标记将有助于分析杂色鲍的种群结构和遗传多样性。