Miralpeix M, Azcon-Bieto J, Bartrons R, Argiles J M
Department de Ciencies Fisiologiques, Humanes i de la Nutricio, Facultat de Medicina, Universitat de Barcelona, Spain.
Cancer Lett. 1990 Apr 30;50(3):173-8. doi: 10.1016/0304-3835(90)90261-u.
Isolated vegetative tumour cells from mice bearing the Lewis lung carcinoma showed low rates of basal respiration with both low oxygen uptake rates and cytochrome-c oxidase activity. The cells were affected by a marked Crabtree effect and a high rate of lactate production in the presence of 10 mM glucose. The glycolytic capacity of the tumour was also assessed through the measurement of the maximum activities for hexokinase, phosphofructokinase, pyruvate kinase and lactate dehydrogenase. These activities were similar to the ones found in other fast-growing, undifferentiated tumours. The concentration of fructose-2,6-bisphosphate in the tumour was 2,3 nmoles/g fresh tissue wt., a value which is of the same order of magnitude as that found in other types of highly glycolytic cells. It is concluded that the Lewis lung carcinoma follows the same pattern as other undifferentiated tumours with a high capacity for both glucose and amino acid utilization.
从携带Lewis肺癌的小鼠中分离出的营养性肿瘤细胞显示出低基础呼吸率，同时氧摄取率和细胞色素c氧化酶活性均较低。在存在10 mM葡萄糖的情况下，这些细胞受到明显的Crabtree效应影响，乳酸产生率很高。还通过测量己糖激酶、磷酸果糖激酶、丙酮酸激酶和乳酸脱氢酶的最大活性来评估肿瘤的糖酵解能力。这些活性与在其他快速生长的未分化肿瘤中发现的活性相似。肿瘤中果糖-2,6-二磷酸的浓度为2.3纳摩尔/克新鲜组织重量，该值与在其他类型的高糖酵解细胞中发现的值处于相同的数量级。得出的结论是，Lewis肺癌与其他未分化肿瘤遵循相同的模式，具有高葡萄糖和氨基酸利用能力。
