Institute of Science, Nirma University, Sarkhej-Gandhinagar Highway, Village - Chharodi, Ahmedabad, Gujarat 382481, India.
J Ethnopharmacol. 2011 Jun 22;136(2):363-7. doi: 10.1016/j.jep.2011.04.066. Epub 2011 May 6.
Eclipta alba is traditionally used as hepatoprotective agent. The study was designed to explore its antiproliferative activity on liver and other related cancer.
The present study was designed to assess and establish the role of Eclipta alba as anti-cancer agent using HepG2, C6 glioma and A498 cell lines as model system.
Antiproliferative and cytotoxic effects of the Eclipta alba hydroalcoholic extract (EAE) was determined using MTT assay. The expression level of NF-kB was analysed by western blotting and RT PCR. Gelatin zymography was done for gelatinase matrix metalloproteinases (MMP-2 and 9) analysis.
EAE inhibited the cell proliferation in dose dependent manner in HepG2, A498 and C6 glioma cell lines with an IC50 of 22±2.9, 25±3.6 and 50±8.7 μg/ml, respectively. The expression of MMP (2 and 9) was down-regulated with EAE treatment. DNA damage was observed following 72h of extract treatment, leading to apoptosis. Additionally, the expression level of NF-kB was evaluated with western blotting and RT-PCR and was found to be down-regulated/inactivated.
The data establish the existence of anti-proliferative, DNA damaging and anti-metastasis properties in EAE which is yet unexplored and hold high therapeutic impact.
旱莲草传统上被用作保肝剂。本研究旨在探索其对肝脏和其他相关癌症的抗增殖活性。
本研究旨在评估和确立旱莲草作为抗癌剂的作用，使用 HepG2、C6 神经胶质瘤和 A498 细胞系作为模型系统。
使用 MTT 测定法测定旱莲草水醇提取物（EAE）的抗增殖和细胞毒性作用。通过 Western blot 和 RT-PCR 分析 NF-κB 的表达水平。进行明胶酶谱分析以分析明胶酶基质金属蛋白酶（MMP-2 和 9）。
EAE 以剂量依赖性方式抑制 HepG2、A498 和 C6 神经胶质瘤细胞系的细胞增殖，其 IC50 分别为 22±2.9、25±3.6 和 50±8.7μg/ml。EAE 处理后 MMP（2 和 9）的表达下调。提取处理 72 小时后观察到 DNA 损伤，导致细胞凋亡。此外，通过 Western blot 和 RT-PCR 评估 NF-κB 的表达水平，发现其被下调/失活。
数据表明 EAE 具有抗增殖、DNA 损伤和抗转移特性，但尚未得到探索，具有很高的治疗影响。
