McNicol P J, Guijon F B, Paraskevas M, Heywood E, Gray M J, Brunham R C
Cadham Provincial Laboratory, Winnipeg, Manitoba, Canada.
Am J Obstet Gynecol. 1990 Apr;162(4):1037-41. doi: 10.1016/0002-9378(90)91311-y.
We observe fluctuations in human papillomavirus detection and variation in genotyping between sequential cervical cell specimens analyzed by filter in situ hybridization. Furthermore, specimen adequacy for analysis varies. To determine whether these phenomena are correlated with menstrual cycle stage at the time of sampling, we analyzed cervical cell specimens from women with cervical intraepithelial neoplasia. Specimens were categorized on the basis of a 28-day menstrual cycle and were analyzed by hybridization to combined probes for virus types 6 and 11 or types 16 and 18. Specimen adequacy was determined by hybridization to a human Alu I repetitive deoxyribonucleic acid probe. Analysis of data with chi 2 revealed that fluctuations in virus detection and type variation are unrelated to menstrual cycle stage. Specimen adequacy is stage-dependent for women who take oral contraceptives. Whereas specimens can be collected at any time other than the first week of the menstrual cycle, accurate determination of infection status requires multiple assessments.
我们观察到，通过滤膜原位杂交分析的连续宫颈细胞标本中，人乳头瘤病毒检测存在波动，基因分型也有差异。此外，用于分析的标本充足性也各不相同。为了确定这些现象是否与采样时的月经周期阶段相关，我们分析了患有宫颈上皮内瘤变的女性的宫颈细胞标本。标本根据28天的月经周期进行分类，并通过与6型和11型或16型和18型病毒的组合探针杂交进行分析。通过与人类Alu I重复脱氧核糖核酸探针杂交来确定标本的充足性。用卡方分析数据表明，病毒检测的波动和类型变异与月经周期阶段无关。对于服用口服避孕药的女性，标本充足性取决于月经周期阶段。虽然可以在月经周期的第一周以外的任何时间采集标本，但准确确定感染状态需要多次评估。
