Fairley C K, Robinson P M, Chen S, Tabrizi S N, Garland S M
Department of Social and Preventive Medicine, Monash University, Alfred Hospital, Prahran, Victoria, Australia.
Genitourin Med. 1994 Jun;70(3):171-4. doi: 10.1136/sti.70.3.171.
To determine if HPV detection or the size of a tampon specimen is affected by the menstrual cycle.
Two hundred and eighty women between 18-35 years of age attending a gynaecology clinic at The Royal Women's Hospital were enrolled. Each woman completed a questionnaire on the risk factors of HPV infection and provided a tampon specimen. Specimens were analysed for the presence of HPV DNA (polymerase chain reaction with the L1 consensus primers) after the pellet volume and number of cells was assessed.
The mean age of the 298 women enrolled in this study was 27.0 years (SD 4.5, range 18-35). Ninety two (30.9%) of the tampon specimens were positive for HPV using the L1 consensus primer. The detection of HPV DNA was not associated with the quartiles of the menstrual cycle (p = 0.32). Both the pellet volume and the number of cells from a tampon specimen were greater during the mid cycle, although this was significant for the pellet volume only (p = 0.002 and 0.1 respectively). The pellet volume was not significantly associated with other variables assessed by the questionnaire. The number of cells from a tampon specimen increased with the numbers of life time sexual partners (p = 0.02) and was higher for a single marital status (p = 0.0008).
The timing of the menstrual cycle effects the size of tampon specimens but not the probability of detecting HPV DNA.
确定月经周期是否会影响人乳头瘤病毒（HPV）检测结果或棉塞样本的大小。
招募了280名年龄在18至35岁之间、前往皇家妇女医院妇科门诊就诊的女性。每位女性填写了一份关于HPV感染风险因素的问卷，并提供了一个棉塞样本。在评估样本沉淀体积和细胞数量后，对样本进行HPV DNA检测（使用L1通用引物的聚合酶链反应）。
参与本研究的298名女性的平均年龄为27.0岁（标准差4.5，范围18 - 35岁）。使用L1通用引物检测时，92个（30.9%）棉塞样本HPV呈阳性。HPV DNA的检测与月经周期的四分位数无关（p = 0.32）。虽然仅沉淀体积在月经周期中期显著更大（分别为p = 0.002和0.1），但棉塞样本的沉淀体积和细胞数量在月经周期中期都更大。沉淀体积与问卷评估的其他变量无显著关联。棉塞样本中的细胞数量随性伴侣数量增加而增多（p = 0.02），且单身婚姻状况下细胞数量更高（p = 0.0008）。
月经周期的时间会影响棉塞样本的大小，但不会影响检测到HPV DNA的概率。
