Pater A, Bayatpour M, Pater M M
Division of Basic Medical Sciences, Faculty of Medicine, Memorial University, Newfoundland, Canada.
Am J Obstet Gynecol. 1990 Apr;162(4):1099-103. doi: 10.1016/0002-9378(90)91323-5.
Compelling evidence supports a role of certain types of human papillomaviruses as the cause of cervical cancer. In addition to human papillomaviruses, other agents, such as hormones, have been implicated as cofactors in this type of neoplasia. In this study we provide evidence for oncogenic transformation of primary baby rat kidney cells by human papillomavirus type 16 deoxyribonucleic acid plus ras oncogene in the presence of progesterone but not estrogen. Integrated and intact human papillomavirus type 16 deoxyribonucleic acid is present and expressed in all the five progesterone-transformed colonies that we examined. Moreover, all these cell lines are capable of anchorage-independent growth and induce tumors in syngeneic animals. We also observed oncogenic transformation with human papillomavirus type 16 deoxyribonucleic acid plus ras in the presence of ethanol-soluble extracts from two brands of commonly used oral contraceptive tablets. No transformation is achieved in the presence of ethanol-soluble extracts from the inert tablets, provided in packages of each brand of oral contraceptive. These results may have implications for a papillomavirus-hormone link to cervical neoplasia.
有力的证据支持某些类型的人乳头瘤病毒是宫颈癌病因这一观点。除人乳头瘤病毒外，其他因素，如激素，也被认为是这类肿瘤形成的辅助因素。在本研究中，我们提供证据表明，在孕酮存在而非雌激素存在的情况下，人乳头瘤病毒16型脱氧核糖核酸加上ras癌基因可使原代新生大鼠肾细胞发生致癌转化。我们检测的所有五个经孕酮转化的菌落中均存在并表达整合且完整的人乳头瘤病毒16型脱氧核糖核酸。此外，所有这些细胞系都能够在不依赖贴壁的情况下生长，并在同基因动物中诱发肿瘤。我们还观察到，在存在两种常用口服避孕药片的乙醇可溶性提取物的情况下，人乳头瘤病毒16型脱氧核糖核酸加上ras可导致致癌转化。而在每个品牌口服避孕药包装中提供的惰性片剂的乙醇可溶性提取物存在的情况下，未实现转化。这些结果可能对乳头瘤病毒与激素在宫颈癌形成中的联系具有启示意义。
