Department of Medicine, Stord Helse-Fonna Hospital, 54 09 Stord, Norway.
Mol Med Rep. 2011 Jul-Aug;4(4):603-5. doi: 10.3892/mmr.2011.492. Epub 2011 May 16.
Microscopic colitis (MC) is a chronic condition that is characterized by watery diarrhoea with normal appearance of the colonic mucosa. MC is subdivided into two distinctive entities: lymphocytic colitis (LC) and collagenous colitis (CC). The etiology and pathophysiology of LC remain to be determined. The present study included 9 female patients with LC, with an average age of 34 years. Subjects (n=25) who underwent colonoscopy were used as controls. The subjects underwent colonoscopy due to gastrointestinal bleeding, where the source of bleeding was identified as haemorrhoids, or due to health concerns. The control subjects included 18 females and 7 males, with an average age of 49 years. Colonoscopy was performed in both patient and control groups, and biopsies were obtained from different segments of the colon. The biopsies were immunostained with the avidin-biotin complex method for human leucocytes CD45, collagen type III and chromogranin A (CgA). CgA was quantified by computer image analysis. The density of CgA-immunoreactive cells in patients with LC was significantly higher than that in controls. The high density of colonic CgA, a common marker for endocrine cells, indicates the possibility that colonic hormones are involved in the pathophysiology of LC. Serotonin-containing cells are the major endocrine cell type in the colon and constitute approximately 88% of the total endocrine cell population. It is likely that the increase in colonic CgA in LC patients accounts for an increase in serotonin cells.
显微镜结肠炎（MC）是一种以水样腹泻为特征且结肠黏膜外观正常的慢性疾病。MC 分为两种独特的实体：淋巴细胞性结肠炎（LC）和胶原性结肠炎（CC）。LC 的病因和发病机制仍有待确定。本研究包括 9 例 LC 女性患者，平均年龄 34 岁。接受结肠镜检查的受试者（n=25）作为对照。受试者因胃肠道出血接受结肠镜检查，出血源确定为痔疮，或因健康问题接受结肠镜检查。对照组包括 18 名女性和 7 名男性，平均年龄 49 岁。对患者组和对照组均进行结肠镜检查，并从结肠的不同部位获取活检。活检采用抗生物素蛋白-生物素复合物方法对人类白细胞 CD45、胶原 III 型和嗜铬粒蛋白 A（CgA）进行免疫染色。通过计算机图像分析对 CgA 免疫反应细胞的密度进行定量。LC 患者结肠 CgA 免疫反应细胞的密度明显高于对照组。富含 CgA 的结肠是肠内分泌细胞的常见标志物，这表明肠激素可能参与 LC 的病理生理学过程。含血清素的细胞是结肠中的主要内分泌细胞类型，约占总内分泌细胞群的 88%。LC 患者结肠 CgA 的增加可能导致血清素细胞的增加。
