Unidad de Biotecnología, Centro de Investigación Científica de Yucatán, Calle 43 No. 130, Colonia Chuburná de Hidalgo, Mérida, Yucatán, 97200, Mexico.
Parasitol Res. 2012 Jan;110(1):31-5. doi: 10.1007/s00436-011-2447-8. Epub 2011 May 17.
Ethanol extracts of Senna villosa, Serjania yucatanensis, Byrsonima bucidaefolia, and Bourreria pulchra were evaluated for their in vitro activity against epimastigotes and trypomastigotes of Trypanosoma cruzi. Results showed that the leaf extracts of S. yucatanensis and B. pulchra were the most active against epimastigotes (IC(100) = 100 μg/mL) and trypomastigotes of T. cruzi (95% or more reduction in the number of parasites at 100 and 50 μg/mL). However, only the leaf extract of S. yucatanensis showed significant trypanocidal activity when tested in vivo, reducing 75% of the parasitemia in infected mice at 100 mg/kg. This same extract inhibited the egress of trypomastigotes from infected cells and proved not to be cytotoxic (IC(50) = 318.8 ± 2.3 μg/mL).
用乙醇提取苦豆草、墨西哥卫矛、南美叶下珠和秀丽粗榧的叶，评估它们对克氏锥虫的滋养体和鞭毛体的体外活性。结果表明，墨西哥卫矛和秀丽粗榧的叶提取物对克氏锥虫的滋养体（IC（100）=100μg/ml）和鞭毛体（在 100 和 50μg/ml 时寄生虫数量减少 95%或更多）最具活性。然而，只有墨西哥卫矛的叶提取物在体内试验中显示出显著的杀锥虫活性，在感染的小鼠中，在 100mg/kg 时将寄生虫血症降低了 75%。该提取物还抑制了从感染细胞中逸出的锥虫，并且没有显示出细胞毒性（IC（50）=318.8±2.3μg/ml）。
