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多态微卫星 DNA 标记在红树蟹 Ucides cordatus(短尾下目:梭子蟹科)中的应用。

Polymorphic microsatellite DNA markers in the mangrove crab Ucides cordatus (Brachyura: Ocypodidae).

机构信息

EMBRAPA Meio-Norte, CP: 01, CEP: 64006-220, Teresina, PI, Brazil.

出版信息

Mol Ecol Resour. 2009 Jul;9(4):1249-52. doi: 10.1111/j.1755-0998.2009.02658.x. Epub 2009 Mar 23.

Abstract

The isolation and characterization of the first polymorphic microsatellite markers for the mangrove crab Ucides cordatus are described. The number of alleles at each locus ranged from three to 25, mean of nine alleles, in 46 crabs captured in two Brazilian mangroves. The markers averaged high levels of observed (0.709  ±  0.183) and expected (0.716  ±  0.170) heterozygosities. Departures from Hardy-Weinberg equilibrium were observed at two loci. Linkage disequilibrium tests were not significant and no evidence of null alleles was detected. All these microsatellite loci are expected to be useful in estimating fine-scale population processes of this valuable mangrove species currently subjected to excessive fishing efforts.

摘要

描述了红树林蟹 Ucides cordatus 第一个多态性微卫星标记的分离和特征。在两个巴西红树林中捕获的 46 只螃蟹中,每个位点的等位基因数从三个到 25 个不等,平均值为 9 个等位基因。标记的观测杂合度(0.709 ± 0.183)和预期杂合度(0.716 ± 0.170)平均值均较高。在两个位点观察到偏离哈迪-温伯格平衡。连锁不平衡测试不显著,也未发现无效等位基因的证据。所有这些微卫星标记预计将有助于估计这种有价值的红树林物种的精细种群过程,目前该物种正受到过度捕捞的影响。

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