小褐果蝠（Eonycteris spelaea）微卫星位点的特征分析。

Characterization of microsatellite loci in the lesser dawn bat (Eonycteris spelaea).

机构信息

Guangdong Entomological Institute, Guangzhou 510260, China, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China, School of Life Science, Guangxi Normal University, Guilin 541004, China, School of Life Science, East China Normal University, Shanghai 200062, China.

出版信息

Mol Ecol Resour. 2008 May;8(3):695-7. doi: 10.1111/j.1471-8286.2007.02051.x.

DOI:10.1111/j.1471-8286.2007.02051.x

PMID:21585874

Abstract

We constructed a genomic DNA library enriched for CA repeat motifs in Eonycteris spelaea. Nine microsatellite loci were isolated and tested on a population of 39 samples from Yunnan Province, China. These nine loci had three to 22 alleles per locus. Observed and expected heterozygosity values ranged from 0.079 to 0.963 and from 0.078 to 0.959. Two loci revealed significant departure from Hardy-Weinberg equilibrium and no linkage disequilibrium was found between loci pairs. These microsatellites can be a powerful molecular tool for population-level studies of E. spelaea.

摘要

我们构建了一个富含中华穿山甲 CA 重复基序的基因组 DNA 文库。从中国云南省的 39 个样本中分离并测试了 9 个微卫星位点。这 9 个位点每个位点有 3 到 22 个等位基因。观测到的和预期的杂合度值范围分别为 0.079 到 0.963 和 0.078 到 0.959。两个位点显示出与哈迪-温伯格平衡显著偏离，并且在位点对之间没有发现连锁不平衡。这些微卫星可以成为中华穿山甲群体水平研究的有力分子工具。